en Biology & physiology Biology & physiology پژوهشي Orginal research papers <span style="font-size:11pt"><span style="line-height:120%"><span style="font-family:Calibri,sans-serif"><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">This study evaluated the healing effect of black seed essential oil (<i>Nigella sativa; </i>NS) dietary supplementation on skin wound in goldfish (<i>Carassius auratus</i>). Some 48 goldfish were divided into 4 groups, including </span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">the control and 3 treatment groups in three replications and kept for study period of 7 days</span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">. NS essential oil was added to the basal diet of </span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">treatment groups </span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">at the concentration of 2% for the NS2 group, 4% for the NS4 group, and 6% for the NS6 group. The control group was fed the basal diet without NS during the experimental period. The 5 mm incision made by scalpel on the right flank of all fishes below the dorsal fin above the lateral line. The fish fed three times a day with assigned diet for a week. Histological investigations approved the effects of NS essential oil on skin wound healing. Diet with 6% concentration of NS6 revealed the improved healing outcomes such as re-epithelialization, muscle regeneration and scale formation rather than NS2 and NS4 groups.</span></span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span> Black seed (Nigella sativa), Carassius auratus, Dietary, Healing, Skin wound 1461 1471 http://jifro.ir/browse.php?a_code=A-10-797-3&slc_lang=en&sid=1 H. Khaleghi khaleghihaniye534@gmail.com 100319475328460046610 100319475328460046610 No DVM Student of Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran A. Sattari amirsatari@uk.ac.ir 100319475328460046611 100319475328460046611 Yes Division of Aquatic Animal Health and Diseases, Department of Food Hygiene and Public health, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran. S. Azizi azizi.shahrzad@gmail.com 100319475328460046612 100319475328460046612 No Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran I. Sharifpour sharifpour@yahoo.com 100319475328460046613 100319475328460046613 No Department of Aquatic Animal Health and Diseases, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran.