en Biotechnology Biotechnology پژوهشي Orginal research papers <span style="font-size:11pt"><span style="line-height:120%"><span style="font-family:Calibri,sans-serif"><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times="">It is believed that the marine animals contain many compounds that could be beneficial for the treatment of many diseases. In this study, the <a name="_Hlk78127765">acetone </a>extract of <i>Stichopus hermanni</i> was fractionated by a liquid chromatography and then fractions were assayed for terpenoids. The fraction C18 that received a positive response for terpenoids was purified further and characterized by the liquid chromatography, mass spectral, and Thin-layer chromatography analysis. A terpenoid compound, squalene, was identified as the constituent of the bioactive extract fraction. Antimicrobial activity of C18-3 was tested against <i>Bacillus subtilis</i>, <i>Bacillus cereus</i>, <i>Pseudomonas aeruginosa</i>, <i>Salmonella typhi</i>, <i>Proteus vulgaris</i>, <i>Escherichia coli</i>, <i>Klebsiella pneumonia</i>, <i>Staphylococcus aureus</i>, and <i>Nocardia brasiliensis</i> pathogens. The lowest MIC and MBC values were observed in the <i>Bacillus cereus</i> (50 &micro;g/mL) and <i>Staphylococcus aureus</i> (500 &micro;g/mL), MIC and MFC the fungal pathogen Candida albicans (400 and 2000&thinsp;&mu;g/mL) and was cytotoxic against KB/ C152 and HUT-78/C185 cells (IC50 6.1 &mu;g/mL and 6.29 &mu;g/mL). This research suggests <i>S. hermanni</i> can be used as an alternative source for the separation and purification of squalene compound as a medicinal supplement.&nbsp;</span></span></span></span></span></span> Antibacterial activity, Anti-fungal activity, Cytotoxic activity, Sea cucumber, Marine natural products 1495 1507 http://jifro.ir/browse.php?a_code=A-10-806-3&slc_lang=en&sid=1 M. Nazemi melikanazemi@yahoo.com 100319475328460046619 100319475328460046619 Yes Persian Gulf and Oman Sea Ecological Research Organization, Iranian Fisheries Research Organization, Bandar Abbas, Iran A. Motallebi abbasalimotallebi@gmail.com 100319475328460046620 100319475328460046620 No Iranian Fisheries Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran E. abbasi elhamabbasi466@yahoo.com 100319475328460046621 100319475328460046621 No Department of Food and Drug Quality Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran M. khaledi mostafakhaledi@outlook.com 100319475328460046622 100319475328460046622 No Marine Pharmaceutical Science Research Center, Department of Pharmacognosy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran. ORCID Id: 0000-0001-7891-2243 M. Zare Md.zare77@gmail.com 100319475328460046623 100319475328460046623 No The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran