en Biotechnology Biotechnology پژوهشي Orginal research papers <a name="_Hlk50742191"></a><span style="font-size:11pt"><span style="line-height:120%"><span style="font-family:Calibri,sans-serif"><a name="_Hlk50742191"><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times="">In the present study, 7% silver-low-density thin polyethylene films were successfully prepared through the melt mixing method. These films were applied to reduce the harmful antibacterial properties of <i>Huso huso </i>fillets at refrigeration temperatures, thus extending their shelf life. The total viable bacteria count for <i>H. huso </i>fillets wrapped in a 7% Ag/LDPE nanocomposite </span></span></span></a><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times="">was 3.04&plusmn;2.43 (log CFU/g) in 10^-2-diluted fish samples, which is significantly lower than those of the control and the LDPE group after two days of cold storage. The lowest </span></span></span><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times="">psychrophilic bacteria count (log </span></span></span><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times="">CFU/g</span></span></span><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times="">) was</span></span></span><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""> recorded for the 10^-2-diluted fish samples encased in Ag/LDPE-NC in which </span></span></span><i><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times="">Staphylococcus aureus</span></span></span></i><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""> count (SAC) reached 1.3&plusmn;1.40 log CFU/g on days 2 and 4 at 10^-2 dilution, respectively. It was extraordinarily going up and reached 6.97&plusmn;3.91 log CFU/g for group Ag/LDPE showing high effectiveness of Ag/LDPE on retarding the SAC sampled from the caviar fish fillets up to the fourth day. It is concluded that application of 7% Ag/LDPE film produced by the melt mixing method can remarkably retard growth of bacteria on fresh <i>H. huso</i> fillets. Thereupon, the total viable bacterial count and psychrophilic bacteria growth and <i>S. aureus</i> activity were significantly mitigated up to four days of preservation at the refrigerator at which the values <span style="background:white"><span style="color:black">were below acceptable limits for fresh fish</span></span></span></span></span><span style="font-size:12.0pt"><span style="background:white"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">.</span></span></span></span></span><span style="font-size:12.0pt"><span style="background:white"><span style="line-height:120%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br> <a name="_Hlk50742191"></a> Nanocomposite, Ag/LDPE, Huso huso, Fillet, Storage 480 499 http://jifro.ir/browse.php?a_code=A-10-2219-6&slc_lang=en&sid=1 M. Yadolahi mahsayadolahi@gmail.com 100319475328460045557 100319475328460045557 No Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran. H. Ahari dr.h.ahari@gmail.com 100319475328460045558 100319475328460045558 Yes Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran. A.A. Anvar saaa4824@gmail.com 100319475328460045559 100319475328460045559 No Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran