Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1401
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gregorian
2022
9
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Research Article: Extraction and investigation of biological activities of dioctyl phthalate and dibutyl phthalate from marine sponge Haliclona (Soestella) caerulea Larak Island, Persian Gulf
Biotechnology
Biotechnology
پژوهشي
Orginal research papers
<span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Marines are unique resource that provides a diverse array of natural products, primarily from invertebrates such as sponge. As infectious diseases evolve and develop resistance to existing pharmaceuticals, these ecosystems provide novel leads against microbial, cancer, and viral diseases. The purpose of this study is to investigate antimicrobial and antifungal activities of derivatives of phthalates extracted from <i>Haliclona</i> </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">(<i>Soestella</i>)<i> </i></span></span></span><i><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">caerulea</span></span></span></i><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> on some pathogenic organisms. S</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">ponge samples from Larak Island were collected by divers at a depth of 10 meters. After that, marine sponge extract collection was done by chloroform-methanol extraction as a solvent and through chromatography column and nuclear magnetic resonance (NMR) spectroscopy on the sponge sample, components of dioctyl phthalate </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">(DOTP)</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> and dibutyl phthalate (DBP) were purified and identified. Anti-microbial and antifungal activity of extracted components were investigated by means of bacterial broth dilution method and microdilution broth method and </span></span></span><span style="color:black"><a href="https://www.google.com/search?sxsrf=ALeKk02EVwE0yEJauODF-S1zQJ29VUGHFQ:1622533263498&q=Minimum+Inhibitory+Concentration+abbreviation&spell=1&sa=X&ved=2ahUKEwiC7bm29_XwAhVPXsAKHVEeCI0QkeECKAB6BAgBEC4"><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="text-decoration:none"><span style="text-underline:none">minimum inhibitor concentration</span></span></span></span></span></a></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> (</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">MIC), </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">minimum bactericide concentration</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> (MBC), and </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">minimum fungal concentration</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> (MFC) were determined. The results indicated that </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">DOTP</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> of <i>H. </i>(<i>Soestella</i>)<i> caerulea</i> was active on <i>Bacillus subtilis</i>, <i>Bacillus cereus</i> bacteria. MBC values of </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">DOTP</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> were determined as 2000 μg/m. DBP of <i>H. </i>(<i>Soestella</i>)<i> caerulea</i> was active on gram positive bacteria. MBC values of </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">DOTP</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> were determined as 1000 μg/m for <i>B. cereus</i> and <i>Staphylococcus aureus</i> and 500 μg/mL for <i>B. subtilis</i>. MFC values of </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">DOTP</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> and DBP were determined as 1000 μg/m and 2000 for <i>Candida albicans</i>. Hence it is assumed that the sponge </span></span></span><i><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Haliclona </span></span></span></i><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">(<i>Soestella</i>)<i> </i></span></span></span><i><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">caerulea</span></span></span></i> <span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">exhibited high antimicrobial activity.</span></span></span></span></span></span></span>
Marine sponge, Extraction, natural compounds, Dibutyl phthalate, Dioctyl phthalate, Antibacterial activity, Antifungal activity, Larak Island.
1141
1155
http://jifro.ir/browse.php?a_code=A-10-1886-4&slc_lang=en&sid=1
E.
Shafeian
elnaz_sh24@yahoo.com
100319475328460046506
100319475328460046506
No
Department of Marine Science, Faculty of Natural resources and Environment, Science and Research Branch, Islamic Azad University, Tehran, Iran
P.
Ghavam Mostafavi
mostafavi_pa@srbiau.ac.ir
100319475328460046507
100319475328460046507
Yes
Department of Marine Science, Faculty of Natural resources and Environment, Science and Research Branch, Islamic Azad University, Tehran, Iran
M.
Moridi Farimani
m_moridi@sbu.ac.ir
100319475328460046508
100319475328460046508
No
Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University
A.
Mashinchian Moradi
ali2m@yahoo.com
100319475328460046509
100319475328460046509
No
Department of Marine Science, Faculty of Natural resources and Environment, Science and Research Branch, Islamic Azad University, Tehran, Iran
M.
Nazemi
melikanazemi@yahoo.co
100319475328460046510
100319475328460046510
No
Persian Gulf and Oman Sea Ecological Research Center, Iranian Fisheries Science Research Institute (IFSRI). Agricultural Research. Education and Extension Organization (AREEO), Bandar Abbas, Iran.