en Biotechnology Biotechnology پژوهشي Orginal research papers This paper focuses on the use of incorporation of silver (Ag) and copper (Cu) nanoparticles in titanium dioxide (TiO<strong>₂</strong>) nanocomposites, as Ag/Cu/TiO<strong>₂</strong> nanocomposite was synthetized through the chemical reduction method against food borne microorganisms. The antimicrobial effect of Ag/Cu/Tio<strong>₂</strong>nanocomposite demonstrated a considerable antimicrobial activity as order as <em>Escherichia coli</em> > <em>Staphylococcus aureus</em> > <em>Aspergillus niger</em>. The results of the agar diffusion test exhibited no halo growth around the discs of four nanocomposite samples, which indicated the perfect inhibitory effect of synthesized Ag/Cu/TiO2 nanocomposites against the afore-mentioned bacteria. Scanning electron microscopy (SEM) analysis exhibited that Ag and Cu nanoparticles were homogeneously distributed into the TiO₂ nanocomposite. Furthermore, the results of inductive coupled plasma mass spectrometry (ICP-MS) method showed that 0/168 ppm of Ag was released to caviar samples on 32nd day and 62nd days of the experiment. All findings indicated that the combined usage of Ag/Cu/Tio₂ nanoparticles could meet all desirable objectives including increase of antimicrobial activity and reduction release of Ag nanoparticles into the caviar, which extends caviar shelf life. Chemical reduction method, Nanocomposites, Shelf life, Caviar, Antimicrobial tests. 13 31 http://jifro.ir/browse.php?a_code=A-10-2219-5&slc_lang=en&sid=1 M. kargar kargarmaryam1010@yahoo.com 100319475328460039160 100319475328460039160 No Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran H. Ahari dr.h.ahari@gmail.com 100319475328460039161 100319475328460039161 Yes Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran Sh. Kakoolaki bsh443@gmail.com 100319475328460039162 100319475328460039162 No Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran M. Mizani Mizani1_2000@yahoo.com 100319475328460039163 100319475328460039163 No Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran