en Genetics Genetics پژوهشي Orginal research papers <a name="OLE_LINK16"></a><a name="OLE_LINK15">A population genetics study on <em>Penaeus semisulcatus </em>from Persian Gulf was performed to assist in the selection of suitable broodstocks for future breeding programs. Eight novel microsatellite loci were developed to study population genetics structure of <em>P. semisulcatus</em> in three population sites, Persian Gulf and Oman Sea (Jask, Hormoz and Kuhestak). There were incidences of heterozygosity deficiency and significant deviations from Hardy Weinberg equilibrium (HWE) at most loci (<em>p</em><0.001). However, four loci (loci E2 and B9 &ndash; Hormoz; loci C6 &ndash; Kuhestak; and loci H9 &ndash; Jask) found to be in HWE. Micro-Checker analysis revealed null alleles in the three microsatellite loci (B5, C6 and C9). Pairwise F_st comparison based on allelic and genotypic frequencies indicated that the three populations were significantly differentiated from each other (<em>p</em><0.05). High levels of pairwise F_st (0.106) and low levels of Nm (2.103) observed between Hormoz and Jask populations indicated restricted gene flow between the two populations. On the other hand, low levels of pairwise F_st (0.016) and high levels of Nm (15.876) observed between Hormoz and Kuhestak populations indicated high gene flow between these populations. In this study, the assignment test was examined in order to find gene flow connectivity between the three populations. Overall, results revealed high gene flow between Hormoz and Kuhestak and restricted genetic flow between Jask and both Hormoz and Kuhestak populations, providing new input for selection of genetically-suitable broodstocks.</a> Penaeus semisulcatus, Genetics, Molecular marker, Polymorphism, Microsatellite 157 178 http://jifro.ir/browse.php?a_code=A-10-2961-1&slc_lang=en&sid=1 S. Tamadoni Jahromi stamadoni@gmail.com 100319475328460039197 100319475328460039197 No Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Abbas, Iran, A.S. Othman sofiman@usm.my 100319475328460039198 100319475328460039198 Yes School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia R. Rosazlina rosazlinarusly@usm.my 100319475328460039199 100319475328460039199 No Centre for Marine and Coastal Studies, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia S. Pourmozaffar sajjad5550@gmail.com 100319475328460039200 100319475328460039200 No Persian Gulf Mollusks Research Station, Agricultural Research Education and Extension Organization (AREEO), Iranian Fisheries Sciences Research Institute, Bandar-e-Lengeh, Iran M. Gozari gozari2020@gmail.com 100319475328460039201 100319475328460039201 No Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Abbas, Iran,