Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1399
8
1
gregorian
2020
11
1
19
6
online
1
fulltext
en
Characterization of Vibrio alginolyticus bacteriophage recovered from shrimp ponds in south west of Iran
aquaculture
aquaculture
پژوهشي
Orginal research papers
Farming of the pacific white leg shrimp (<em>Penaeus vannamei</em>), consists the greatest percent of aquaculture production of crustaceans all over the world. Vibriosis caused by <em>Vibrio </em>species such as <em>Vibrio alginolyticus</em>, is a serious limiting agent for aquaculture. Phage therapy is an eco-friendly alternative to antibiotics as a prophylactic measure for the growth of pathogenic bacteria. The aim of this study was isolation and characterization of <em>Vibriophages</em> present in <em>Litopenaeus vannamei</em> breeding and culture facilities and estuarine resources in south west of Iran, Choebdeh shrimp farming site of Abadan city, Khuzestan Province, based on morphological structure, biological conditions and their potential applications in lysis of <em>Vibrio</em> bacteria. The bacterial and phage samples were analyzed using different physicochemical parameters, 16S rRNA gene sequencing and TEM. The results showed the <em>V. alginolyticus</em> isolates and identified the <em>Podoviridae</em> family of phages. These bacterial isolates were resistant to ampicillin and penicillin. The efficacy of the <em>Podoviridae</em> phage against <em>V. alginolyticus </em>bacterium indicated that this phage has a short period of 30 minutes latent phase, possessed a desirable stability and growth in keeping for 1 hour at 30-55<sup>o</sup>C, the salinity of 0.5-10 <img alt="" chromakey="white" src="file:///C:UsersR8623~1.FALAppDataLocalTempmsohtmlclip1�1clip_image001.png" > and pH of 5-8. Based on evaluation of phage therapy, mean absorbance values (OD<sub>600</sub>), in control and treatment groups, at 12 and 24 hours after phage inoculation, were significantly different (<em>p</em><0.05). According to the results, phage therapy with single-phage suspensions did not prevent bacterial regrowth after treatment. However, delay in development of resistance is a desirable success.
Penaeus vannamei, V. alginolyticus, Choebdeh Abadan, SPSPW phage, Phage therapy
3183
3200
http://jifro.ir/browse.php?a_code=A-10-3523-2&slc_lang=en&sid=1
S.
Rezaee
saiwan_rezaei@yahoo.com
100319475328460038398
100319475328460038398
No
Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
E.
Rajabzadeh Ghatrami
Rajabzadeh48@gmail.com
100319475328460038399
100319475328460038399
Yes
Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
H.
Farahmand
hfarahmand@ut.ac.ir
100319475328460038400
100319475328460038400
No
Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
E.
Zamani
issac.zfr@gmail.com
100319475328460038401
100319475328460038401
No
Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran