Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1397
10
1
gregorian
2019
1
1
18
1
online
1
fulltext
other
Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay
پژوهشي
Orginal research papers
<a>Caspian trout (<em>Salmo trutta caspius</em> Kessler, 1877) as endemic migratory fish, possessing great ecological economic and biodiversity value. Among the habitat drivers, temperature related effects on the genome were considered in this research. During the embryonic developmental stages, genomic alterations of single cells were assessed by Comet assay. After artificial propagation of wild broodstocks, the batches of eggs, were transferred to incubators with constant temperatures, REDD water recycling, 3 stages water filtration, UV-C application on effluent of each incubator and DO–pH–temperature control. Samples were collected from normal (8°C), cold (4°C) and warm (12°C) treatments on eyed egg, alevin and larval stages. Single Cell Gel Electrophoresis were applied on samples for evaluation of Comet Tail length (TL), DNA in tail (DT) and Tail Moment (TM) indices as DNA damage markers. Comparison between mean values of Comet TL index indicated 85.19 ±9.2, 9.88 ±2.3 and 256.54±14.6 for 4, 8 and 12°C treatments, respectively. DT were 18.07±6.3, 9.72±1.9 and 31.47±2.8 and then TM were measured as 15.27± 4.1, 1.21±0.2 and 95.25±8.6 for 4, 8, and 12°C treatments, respectively. Significant difference between thermal treatments (<em>p</em><0.05) observed. Warm treatments demonstrated greater DNA damage in compare with others. TM explained better information in comparison with TL and DT in all analyzed samples. In conclusion, temperatures out of the range of the optimal conditions could significantly affect Caspian trout genome which could be the subject of management considerations pertaining to Caspian trout stocks rehabilitation and captive rearing strategies.</a><a href="#_msocom_1" id="_anchor_1" name="_msoanchor_1" uage="JavaScript">[PN1]</a>
<div>
<hr align="left" size="1" width="33%" >
<div><div id="_com_1" uage="JavaScript"></div></div>
<div></div></div>
Caspian trout, Salmo trutta caspius, Temperature, DNA damage, Comet assay
48
59
http://jifro.ir/browse.php?a_code=A-10-225-4&slc_lang=other&sid=1
H.R.
Alizadeh Sabet
100319475328460029761
100319475328460029761
No
Coldwater Fishes Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Training and Extension Organization ,Tonekabon, Iran
Coldwater Fishes Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Training and Extension Organization ,Tonekabon, Iran
M.R.
Kalbassi
kalbassi_m@modares.ac.ir
100319475328460029762
100319475328460029762
Yes
Department of Aquaculture, Tarbiat Modares University, Faculty of Marine Sciences, Noor, Iran
Department of Aquaculture, Tarbiat Modares University, Faculty of Marine Sciences, Noor, Iran
M.
Pourkazemi
100319475328460029763
100319475328460029763
No
Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Training and Extension Organization, P.O. Box14155-6116, Tehran, Iran.
Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Training and Extension Organization, P.O. Box14155-6116, Tehran, Iran.
M.
Sadeghizadeh
100319475328460029764
100319475328460029764
No
Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran