en Biotechnology Biotechnology پژوهشي Orginal research papers All <em>Spatoglossum</em> and <em>Stoechospermum</em> species in Persian Gulf and Oman &nbsp;Sea, Iran, have been so far identified via classical taxonomy. In this study classification of two genus of brown algae (Dictyotaceae) including <em>Spatoglossum</em> and <em>Stoechospermum</em> have been investigated. We combined the cytoplasmic DNA sequences data of plastid<em> rbc</em>L and <em>psbA</em> with morphological information. Based on the constructed phylogenetic trees on the sequences data of these two genes the collected specimens from the Iranian coastlines made two distinct clades which were grouped with two species <em>Stoechospermum polypodioides</em> and <em>Spatoglossum crassum</em> with high to full bootstrap values. So we approved the presence of <em>Stoechospermum polypodioides</em> and &nbsp;<em>Spatoglossum crassum</em> as new record for the algal flora of Persian Gulf in Iranian coastlines.<span dir="RTL"></span> Dictyotales, Diversity, DNA barcoding, psbA, rbcL, Spatoglossum crassum, Stoechospermum polypodioides 2560 2572 http://jifro.ir/browse.php?a_code=A-10-3126-1&slc_lang=en&sid=1 M. Sadeghi mahnaz.sadeghi66@gmail.com 100319475328460036428 100319475328460036428 No Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol 98613-35856, Iran B.A. Fakheri ba_fakheri@yahoo.com 100319475328460036429 100319475328460036429 Yes Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol 98613-35856, Iran J. Sohrabipour sohrabipour2@gmail.com 100319475328460036430 100319475328460036430 No Department of Natural Resources, Agricultural and Natural Resources Research and Education Center of Hormozgan, Bandar abbas, Iran A. Emamjomeh aliimamjomeh@alumni.ut.ac.ir 100319475328460036431 100319475328460036431 No Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol 98613-35856, Iran A.N. Bagheri nabibagheri@yahoo.com 100319475328460036432 100319475328460036432 No Department of Natural Resources, Agricultural and Natural Resources Research and Education Center of Hormozgan, Bandar abbas, Iran D. Samsampour samsampoor@bmn.ir 100319475328460036433 100319475328460036433 No Department of Horticultural Sciences, Faculty of Agriculture, University of Hormozgan, Iran