Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1399
2
1
gregorian
2020
5
1
19
3
online
1
fulltext
en
DNA barcoding of Nereididae polychaetes based on COI gene barcoding in intertidal shores of Bushehr and
Bandar Abbas provinces, Iran
Genetics
Genetics
پژوهشي
Orginal research papers
Although polychaetes are the most abundant organisms in marine ecosystems, still their genetic diversity is understood inadequately. In this study, molecular identification of Nereididae polychaetes was performed by sequencing a segment of mitochondrial COI gene, isolated from mitochondrial DNA, and comparing nucleotide divergence, Molecular taxonomy, interspecific and intraspecific relations of Nereid species among 4 intertidal stations assigned in Persian Gulf of Iran. Analysis of 109 identified specimens, revealed 78 provisional gene sequences, related to 9 species and 6 genera, in which interspecific divergence was 1.4 times higher than intraspecific divergence (2.82% versus 1.95%). The average pairwise sequence divergence for all sequences was estimated at 1.37%. In three cases maximum divergence within a lineage exceeded the minimum nearest-neighbor distance: <em>Perinereis</em> sp., <em>Platynereis</em> sp. and <em>Platynereis</em> <em>bicanaliculata</em><span dir="RTL">. </span>Maximum species similarity was observed amongst 3 sampling sites assigned in Bushehr Province whilst Bandar Abbas`s specimens showed less similarity to Bushehr station. Out of the 109 COI gene sequences of Nereididae polychaetes in this study, 34 contained multiple lineages<span dir="RTL">. </span>These results support the assertion that many Nereid populations in the Persian Gulf previously thought of as a single species, actually consist of two or more divergent lineages.
Polychaetes, Nereididae, COI, Barcoding, Persian Gulf
1193
1213
http://jifro.ir/browse.php?a_code=A-10-433-2&slc_lang=en&sid=1
S.
Amiri
amiri.sara.ir@gmail.com
100319475328460034033
100319475328460034033
No
Marine Biology Group, Marine Science and Technology Department, Islamic Azad University Science and Research Branch, Tehran, Iran.
P.
Ghavam Mostafavi
mostafavi_pa@srbiau.ac.ir
100319475328460034034
100319475328460034034
Yes
Marine Biology Group, Marine Science and Technology Department, Islamic Azad University Science and Research Branch, Tehran, Iran.
M.B.
Nabavi
m.nabavi@kmsu.ac.ir
100319475328460034035
100319475328460034035
No
Marine Biology Group, Oceanic and Marine Science Department, Khorramshahr Marine Science and Technology University, Khorramshahr, Iran
M.H.
Shahhosseini
shahhosseiny@yahoo.com
100319475328460034036
100319475328460034036
No
Islamic Azad University Shar-e-Qods Branch, Qods, Iran.