Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1385
10
1
gregorian
2007
1
1
6
2
online
1
fulltext
en
tudy on sex steroid-binding proteins (with emphasize on 17 -estradiol) in plasma of female and juvenile kutum (Rutilus frisii kutum)
Biotechnology
Biotechnology
پژوهشي
Orginal research papers
A sex steroid-binding protein (SBP) that binds to 17 b-estradiol with high affinity and moderate capacity was identified in the plasma of female and juvenile of kutum (<em>Rutilus frisii kutum</em>) sampled during the early stage of gonadal maturation in October and prior to spawning in March. Mean maximum specific binding (<em>B</em><sub>max</sub>) and equilibrium dissociation constant (<em>K</em><sub>d</sub>) of the fish were as follows: In early stage of gonadal development (October), <em>B</em><sub>max</sub>= 5800±970 fmol <em>E</em><sub>2</sub>/m gprotein, <em>K</em><sub>d</sub> 4.1±0.6nM, and prior to spawning (March) <em>B</em><sub>max</sub>= 4000±895 fmol <em>E</em><sub>2</sub>/mgprotein, <em>K</em><sub>d</sub> 2.9±0.3nM. In juvenile sampled in October <em>B</em><sub>max</sub>= 1600±130 fmol <em>E</em><sub>2</sub>/mg protein, <em>K</em><sub>d</sub> 2.1±0.2n M and in March samples <em>B</em><sub>max</sub>= 3500±780 fmol E<sub>2</sub>/mg protein, <em>K</em><sub>d </sub>2.2±0.2nM. Plasma estradiol binding characteristics of the adult samples in October and March differed from the juvenile samples in having both <em>B</em><sub>max </sub>and <em>K</em><sub>d</sub> significantly higher than juveniles. Plasma SBP levels displayed a moderate correlation with GSI (<em>r</em><sup>2</sup>= 0.52) and CF (<em>r</em><sup>2</sup>= 0.51) and a weak correlation with HSI (<em>r</em><sup>2</sup> = 0.28). Affinity was moderately correlated with CF (<em>r</em><sup>2</sup> = 0.68) and HIS (<em>r</em><sup>2</sup> = 0.50). A strong correlation was obtained between <em>B</em><sub>max</sub> and <em>K</em><sub>d</sub>, high <em>B</em><sub>max </sub>values coincided with high <em>K</em><sub>d</sub> values and vice versa.
17 -estradiol, Plasma, Sex steroid-binding protein, Kutum
77
92
http://jifro.ir/browse.php?a_code=A-10-600-113&slc_lang=en&sid=1
A.
Kousha
armin_kousha@hotmail.com
100319475328460024268
100319475328460024268
Yes
F.
Asgarian
100319475328460024269
100319475328460024269
No
H.V.
Ghate
100319475328460024270
100319475328460024270
No
V.S.
Ghole
100319475328460024271
100319475328460024271
No