Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1398
12
1
gregorian
2020
3
1
19
2
online
1
fulltext
en
Isolation and characterization of acid-soluble collagen from the skin of Rutilus frisii kutum (Kamensky) of
the Caspian Sea
Biotechnology
Biotechnology
پژوهشي
Orginal research papers
This study proceeds to extract collagen from the skin of <em>Rutilus kutum</em>, one of the main and economical species of the Caspian Sea. Acid-soluble collagen (ASC) from the skin of <em>R. kutum</em> was extracted and characterized by SDS-PAGE, FTIR and UV spectrophotometry. Based on the data from the SDS-PAGE and the composition of amino acid, it was probable that the obtained collagen classified as type I collagen. Collagen consists of two different types of α chains, including α<sub>1 </sub>and α<sub>2</sub>, and β components. Also, FTIR analysis revealed the presence of the helical arrangements of the collagen. Moreover, UV spectrophotometry exhibited this collagen has an absorbance at 220 nm. On the basis of dry weight, the yield of ASC was calculated as 15.6%. The amount of proline in ASC was 89.6 residues per 706.1 residues, and 25.7% of the collagen was composed of glycine. These results showed that <em>R. Kutum</em> skin could be considered as a possible source of collagen and can supply the theoretical basis for further research.
Rutilus Kutum, Fish skin collagen, Characterization, Amino acid composition
768
779
http://jifro.ir/browse.php?a_code=A-10-2359-1&slc_lang=en&sid=1
S.
Naderi Gharagheshlagh
soheila_naderi2@yahoo.com
100319475328460034778
100319475328460034778
No
1. Department of Marine Biology, Science and Research Branch, Islamic Azad university, Tehran, iran.
M.J.
Fatemi
mjfatemi41@gmail.com
100319475328460034779
100319475328460034779
No
Plastic & Reconstructive Surgery Department, Burn Research Center, Tehran, Iran. and 6. University of Medical Sciences, Tehran, Iran.
Sh.
Jamili
shahlajamili45@yahoo.com
100319475328460034780
100319475328460034780
Yes
1. Department of Marine Biology, Science and Research Branch, Islamic Azad university, Tehran, iran. and 5. Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran.
A.M.
Nourani
sharifalim@gmail.com
100319475328460034781
100319475328460034781
No
3. Tissue Engineering Group (TEG) and Research, National Orthopedic Centre of Excellence in Research and Learning (NOCERAL), Department of Orthopedics, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia and Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran.
M.R.
Nourani
nmr41@yahoo.com
100319475328460034782
100319475328460034782
No
4. Division of Genomics, Baqiyatallah University of Medical Sciences.