Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1395
7
1
gregorian
2016
10
1
15
4
online
1
fulltext
fa
Transcriptome analysis of the freshwater pearl mussel, Hyriopsis cumingii (Lea) using illumina paired-end sequencing to identify genes and markers
aquaculture
aquaculture
پژوهشي
Orginal research papers
<p>The transcriptome of triangle sail mussel <em>Hyriopsis cumingii</em> (Lea) using Illumina paired-end sequencing technology was conducted and analyzed. Equal quantities of total RNA isolated from six tissues, including gonad, hepatopancreas, foot, mantel, gill and adductor muscle, were pooled to construct a cDNA library. A total of 58.09 million clean reads with 98.48 % Q20 bases were generated. Clustering and assembling of these reads produced a non-redundant set of 92,347 unigenes with an average length of 1,150.61 bp. 11,174 unigenes were involved in the molecular function, cellular component and biological process categories by GO Gene Ontology)analysis. Potential genes and their functions were predicted by KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway mapping and COG(Cluster of Orthologous Groups of proteins)analysis. More than 8 putative genes of interest involved in sex determination/differentiation were identified. Furthermore, 8,014 SSRs and 38,288 SNPs were identified in this transcriptome dataset.</p>
Hyriopsis cumingii, Illumina paired-end sequencing, Transcriptome, Sex determination, Molecular marker
1425
1440
http://jifro.ir/browse.php?a_code=A-10-600-51&slc_lang=fa&sid=1
A.J.
Zhang
100319475328460019950
100319475328460019950
No
S.L.
Liu
100319475328460019951
100319475328460019951
No
J.Y.
Zhu
100319475328460019952
100319475328460019952
No
Z.M.
Gu
100319475328460019953
100319475328460019953
No
Z.M.
Zhou
100319475328460019954
100319475328460019954
No
G.F.
Zhang
100319475328460019955
100319475328460019955
No
K.H.
Lu
lukaihong@nbu.edu.cn
100319475328460019956
100319475328460019956
Yes