en Biochemistry Biochemistry پژوهشي Orginal research papers <p>Ambient temperature is one of the most important environmental factors affecting physiological mechanisms and biochemical reactions of living organisms. Thus the effect of ambient temperature on HSPs and IGF-I gene expression levels in the liver and muscle tissues of <em>Sparus aurata </em>were investigated in this research. The levels of HSPs, and IGF-I gene expression of the liver and muscle of <em>Sparus aurata</em> were analyzed in by qRT-PCR. The experiment was done&nbsp;&nbsp; in July (27 ^◦C) and&nbsp; January (18^◦C). HSP70 mRNA relative expression levels in the muscle on January were significantly higher than July (approximately 1.7 fold), whereas HSP30 gene expression in the liver on July was increased by 2.0 fold (<em>p</em><0.05). Transcription of other heat shock proteins and IGF-I were not affected by water temperature changing. The HSP findings of the research show that these proteins are important and sensitive in the average adaptation.</p> Sparus aurata, Temperature, IGF-I, HSPs, Gene expression 1014 1024 http://jifro.ir/browse.php?a_code=A-10-2243-1&slc_lang=en&sid=1 A. Bildik bildik65@hotmail.com 100319475328460031074 100319475328460031074 Yes Adnan Menderes University, Veterinary Medicine Faculty, Biochemistry Department, Aydın/TURKEY G.S. Asıcı Ekren gmz-svr@hotmail.com 100319475328460031075 100319475328460031075 No Adnan Menderes University, Veterinary Medicine Faculty, Biochemistry Department, Aydın/TURKEY G. Akdeniz gamze_tosun88@hotmail.com 100319475328460031076 100319475328460031076 No Ministy of Customs and Trade, Izmir/TURKEY F. Kıral fkargin@hotmail.com 100319475328460031077 100319475328460031077 No Adnan Menderes University, Veterinary Medicine Faculty, Biochemistry Department, Aydın/TURKEY