Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1398
4
1
gregorian
2019
7
1
18
3
online
1
fulltext
en
Comparison of the adjuvant effect of propolis and Freund on the efficacy of Aeromonas hydrophila vaccine in
common carp (Cyprinus carpio)
fish disease
fish disease
پژوهشي
Orginal research papers
The success of many vaccines relies on their association with selected adjuvants in order to increase their immunogenicity and ensure long-term protection. Propolis is a natural compound, mostly known for its immunostimulatory properties. In this study the adjuvant effects of propolis in combination with formalin-killed <em>Aeromonas hydrophila</em> vaccine in Common carp (<em>Cyprinus carpio</em>) were evaluated and compared with Freund’s adjuvant. Three hundred juvenile carp (weighing 50.4±3.2 g) were divided randomly into four groups in triplicates. The fish were intraperitonealy injected with <em>A. hydrophila</em> bacterin (A.h) in combination with normal saline (Formalin Killed Bacterin, FKB group), Freund (FKB +F group), and Propolis (FKB +P group) respectively. The control group was injected with normal saline. Serum samples were taken from fish in each group every other week (days 0, 14, 28 and 42) of the experiment and immunological parameters including anti <em>A.</em><em> hydrophila</em> antibody titer, serum lysozyme and antibacterial activity, complement activity, Nitro blue tetrazolium activity and serum total protein and globulin were compared among the groups. At the end of study the remaining fish in each group were challenged with virulent <em>A. hydrophila</em> and mortality was recorded for 10 days and Relative Percentage Survival (RPS) was calculated and compared among the groups. Results showed that although antibody titer and most of none specific immune responses increased in groups 2 and 3 compare to control group (<em>p</em><0.05), no significant difference were seen in group 1 and 3 (<em>p</em>>0.05). Besides no significant change was observed in mortality rate following the challenge in the propolis adjuvanted group compared with the FKB group (<em>p</em>>0.05). According to results, it can be concluded that although propolis as an adjuvant can promote some immune responses of common carp, it can't affect efficacy of vaccine and Ab titer of injected antigens, so it seems more work is needed to present propolis as a proper candidate for the development of a natural adjuvant in fish vaccines.<br>
Propolis, Common carp, Adjuvant, Aeromonas hydrophila, Freund adjuvant, Killed vaccine
428
444
http://jifro.ir/browse.php?a_code=A-10-792-4&slc_lang=en&sid=1
M.
Alishahi
alishahim@scu.ac.ir
100319475328460030287
100319475328460030287
Yes
Department of Clinical sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz
M.
Tollabi
mortezatollabymtm@yahoo.com
100319475328460030288
100319475328460030288
No
Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz
M.
Ghorbanpour
ghorbanpoor2000@yahoo.com
100319475328460030289
100319475328460030289
No
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz