RT - Journal Article T1 - Research Article: Extraction and characterization of collagen from the skin and bone of shabout (Arabibarbus grypus Heckel, 1843) JF - IFRO YR - 2022 JO - IFRO VO - 21 IS - 3 UR - http://jifro.ir/article-1-4669-en.html SP - 671 EP - 687 K1 - Shabout (Arabibarbus grypus) K1 - Characterization K1 - FTIR K1 - SEM skin K1 - bone K1 - Type I collagen AB - Acid soluble collagen (ASC) from the skin (ASC-S) and bone (ASC-B) of shabout (Arabibarbus grypus Heckel, 1843) were isolated and characterized. Both ASC-S and ASC-B from shabout contained glycine as the major amino acid and high amount of proline (Pro), hydroxyproline (Hyp), alanine and glutamic acid. On the basis of dry weight, yields of ASC-S and ASC-B were 6.79 and 2.57%, respectively. Furthermore, fourier transform infrared spectroscopy (FTIR) proved that both collagens were integrated and native. The denaturation temperature of ASC-S and ASC-B were 31.59 and 32.25°C, respectively. Additionally, the results of X-ray diffraction (XRD) proved that the two products retained their helical structures. These collagens had prominent absorptions at 230 nm by UV-Vis spectra. Additionally, the scanning electron microscope (SEM) studies have shown that ACS-S and ASC-B are porous and exhibited fibrous nature. According to the UV–Vis and FTIR results, extracted collagens were characterized as Type I collagen based on their amino acid profile. In the current study, the total amount of amino acid (Pro+Hyp) was 19.28% and 19.55% for ASC-S and ASC-B, respectively. The results of the current study suggested that the collagen isolated from shabout can potentially be an alternative source for use in the food, pharmaceutical and biomedical industries. LA eng UL http://jifro.ir/article-1-4669-en.html M3 ER -