%0 Journal Article %A Jing, Zhou %A Junpeng, Cai %T Isolation, identification and phylogenetic analysis of a pathogen of Haliotis diversicolor supertexta (L.) with mass mortalities %J Iranian Journal of Fisheries Sciences %V 12 %N 4 %U http://jifro.ir/article-1-1344-en.html %R %D 2013 %K Haliotis diversicolor supertexta, massive death, challenge test, Oceanomonas doudoroffii, 16S rDNA sequencing, %X This study was conducted to determine a disease outbreak in 14 day old post-larvae of abalone (Haliotis diversicolor supertexta) which caused mass mortality in July 2010 in Shanwei, China. Twenty-nine bacterial strains were isolated from a sample pool of 10 diseased post-larval abalones on 2216E marine agar plates during a natural outbreak of the disease. Among them, a dominant isolate (referred to as strain 21) was found to be highly virulent to post-larvae in experimental challenge tests, with an LD50 value of 1.0 ×104 colony forming units (CFU) mL-1 on day 3. API 20NE kits and 16S rDNA sequence analysis, identified strain 21 as Oceanomonas doudoroffii. It was susceptible to 10 and moderately susceptible to 1 of the 16 antibiotics examined when antibiotic sensitivities of the bacterium were assayed. Results of this study implicated Oceanomonas doudoroffii strain 21 as a cause of mortalities in post-larval abalone from Shanwei, China. %> http://jifro.ir/article-1-1344-en.pdf %P 843-854 %& 843 %! %9 Orginal research papers %L A-10-1272-46 %+ %G eng %@ 1562-2916 %[ 2013