[صفحه اصلی ]   [Archive]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
:: دوره 20، شماره 2 - ( 1399 ) ::
جلد 20 شماره 2 صفحات 343-357 برگشت به فهرست نسخه ها
Research Article: Characterization of a high-affinity polyclonal antibody against the Cyprinus carpio (Linnaeus, 1758) intestinal tight junction protein occluding
چکیده:   (199 مشاهده)
Tight junction protein, occludin, plays an important role in intestinal health of fish. To study the function of occludin in the intestinal barrier at the protein level, a rabbit occludin polyclonal antibody was prepared against heterologously expressed Cyprinus carpio A fragment of the occludin gene containing antigenic determinants was first ligated into the pET-21a, which is an expression vector and transformed into E. coli BL21 (DE3) strain. Expression of the target fusion protein was induced by isopropyl-beta-D-thiogalactopyranoside (IPTG). The purified fusion protein was used as an antigen to immunize New Zealand long-eared rabbits (Oryctolagus cuniculus) through ear margin vein and subcutaneous injection to obtain rabbit anti-carp polyclonal antibodies against occludin. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the antibody titre, and the antibody was used to determine the distribution and expression of occludin in the intestine of carp after infection with Aeromonas hydrophila. The target fusion protein had a molecular weight of approximately 31.7 ku, the antibody titre was 2.4 × 106, and the integrity of occludin protein was lower in various intestinal segments after A. hydrophila infection. The results indicated that the prepared antibody had a high titre, affinity, and specificity and can be applied to study the expression and distribution of occludin in C. carpio. The availability of this polyclonal antibody laid the foundation for the systematic study of the intestinal barrier of Cyprinus carpio . Additionally, this polyclonal antibody could also be used for explorative studies of the biological function of occludin in other fishes.
متن کامل [PDF 480 kb]   (56 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: aquaculture
دریافت: 1397/4/12 | پذیرش: 1398/7/24 | انتشار: 1399/12/30
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA


XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Feng J, Guo X, Chang X, Zhang Y, Meng X, Lu R, et al . Research Article: Characterization of a high-affinity polyclonal antibody against the Cyprinus carpio (Linnaeus, 1758) intestinal tight junction protein occluding. IJFS. 2021; 20 (2) :343-357
URL: http://jifro.ir/article-1-3501-fa.html

Research Article: Characterization of a high-affinity polyclonal antibody against the Cyprinus carpio (Linnaeus, 1758) intestinal tight junction protein occluding. مجله علوم شیلاتی ایران. 1399; 20 (2) :343-357

URL: http://jifro.ir/article-1-3501-fa.html



دوره 20، شماره 2 - ( 1399 ) برگشت به فهرست نسخه ها
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.04 seconds with 30 queries by YEKTAWEB 4297