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:: دوره 19، شماره 3 - ( 1399 ) ::
جلد 19 شماره 3 صفحات 1384-1395 برگشت به فهرست نسخه ها
Phylogenetic analysis of viral hemorrhagic septicemia virus from recent outbreaks in some of the farmed rainbow trout, Oncorhynchus mykiss (Walbaum, 1792)
چکیده:   (1000 مشاهده)
During a fish disease outbreaks in 2015-2016 suspected to viral hemorrhagic septicemia virus (VHSV), moribund rainbow trout fries were obtained from fish farms. Samples of kidney, heart and spleen tissues were taken  for examination. The full coding region of the G gene was amplified by RT-PCR reaction. Genotyping was carried out by phylogenetic analysis with reference sequences of four VHS genogroup. A phylogenetic tree was constructed with the maximum likelihood method and the Kumara 2- parameter substitution model by using MEGA software version 6.06. A maximum likelihood phylogenetic analysis reveals that all the Iranian VHS   strains belonged to clade Ia-2 of genotype Ia. The intra- sequence comparison of Iranian VHS strains ranged from 99.1% to 100% (average was 99.55%). In this study, two strains (Chaharmahal and Bakhtiari (1) and Chaharmahal and Bakhtiari (3) showed 100% identity but 0.1% differences with Isfahan strain. The Iranian strains belonging to European genogroup are related (99% identity) to VHSV from recent Italian strains (VHSV/O.mykiss/I/TN/133/Apr10).

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نوع مطالعه: پژوهشي | موضوع مقاله: fish disease
دریافت: 1396/7/29 | پذیرش: 1397/3/19 | انتشار: 1399/2/12
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Fattahi F, Akhlaghi M, Mohammadi A, Soltanian S, Shahbazian N. Phylogenetic analysis of viral hemorrhagic septicemia virus from recent outbreaks in some of the farmed rainbow trout, Oncorhynchus mykiss (Walbaum, 1792). IJFS. 2020; 19 (3) :1384-1395
URL: http://jifro.ir/article-1-2994-fa.html

Phylogenetic analysis of viral hemorrhagic septicemia virus from recent outbreaks in some of the farmed rainbow trout, Oncorhynchus mykiss (Walbaum, 1792). مجله علوم شیلاتی ایران. 1399; 19 (3) :1384-1395

URL: http://jifro.ir/article-1-2994-fa.html



دوره 19، شماره 3 - ( 1399 ) برگشت به فهرست نسخه ها
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
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