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Research Article: Extraction and characterization of collagen from the skin and bone of shabout (Arabibarbus grypus Heckel, 1843)
M. Göçer *
Department of Aquaculture and Fisheries Program, Kahta Vocational Training School, Adıyaman University, Adıyaman, Turkey , mmgocer@gmail.com
Abstract:   (568 Views)

Acid soluble collagen (ASC) from the skin (ASC-S) and bone (ASC-B) of shabout (Arabibarbus grypus Heckel, 1843) were isolated and characterized. Both ASC-S and ASC-B from shabout contained glycine as the major amino acid and high amount of proline (Pro), hydroxyproline (Hyp), alanine and glutamic acid. On the basis of dry weight, yields of ASC-S and ASC-B were 6.79 and 2.57%, respectively. Furthermore, fourier transform infrared spectroscopy (FTIR) proved that both collagens were integrated and native. The denaturation temperature of ASC-S and ASC-B were 31.59 and 32.25°C, respectively. Additionally, the results of X-ray diffraction (XRD) proved that the two products retained their helical structures. These collagens had prominent absorptions at 230 nm by UV-Vis spectra. Additionally, the scanning electron microscope (SEM) studies have shown that ACS-S and ASC-B are porous and exhibited fibrous nature. According to the UV–Vis and FTIR results, extracted collagens were characterized as Type I collagen based on their amino acid profile. In the current study, the total amount of amino acid (Pro+Hyp) was 19.28% and 19.55% for ASC-S and ASC-B, respectively. The results of the current study suggested that the collagen isolated from shabout can potentially be an alternative source for use in the food, pharmaceutical and biomedical industries.

Keywords: Shabout (Arabibarbus grypus), Characterization, FTIR, SEM skin, bone, Type I collagen
Full-Text [PDF 862 kb]   (288 Downloads)    
Type of Study: Orginal research papers | Subject: aquaculture
Received: 2021/03/31 | Accepted: 2022/04/4 | Published: 2022/05/20

 


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Göçer M. Research Article: Extraction and characterization of collagen from the skin and bone of shabout (Arabibarbus grypus Heckel, 1843). IJFS. 2022; 21 (3) :671-687
URL: http://jifro.ir/article-1-4669-en.html


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Volume 21, Issue 3 (2022) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
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