en Fish processing Fish processing پژوهشي Orginal research papers <span lang="TR" style="font-size:11.0pt"><span style="font-family:&quot;Calibri&quot;,sans-serif"><span style="color:black">This study aimed to create a novel biodegradable film by incorporating biosynthesized titanium dioxide (TiO₂) nanoparticles into a zein/fucoidan matrix for food packaging applications. TiO</span></span></span><span lang="TR" style="font-size:11.0pt"><span style="font-family:&quot;Cambria Math&quot;,serif"><span style="color:black">₂</span></span></span><span lang="TR" style="font-size:11.0pt"><span style="font-family:&quot;Calibri&quot;,sans-serif"><span style="color:black"> nanoparticles were synthesized using <i>Sargassum tenerrimum</i> extract and confirmed using UV-Vis spectroscopy and dynamic light scattering (DLS). The antioxidant activity was assessed by DPPH assay, showing an IC</span></span></span><span lang="TR" style="font-size:11.0pt"><span style="font-family:&quot;Cambria Math&quot;,serif"><span style="color:black">₅₀</span></span></span><span lang="TR" style="font-size:11.0pt"><span style="font-family:&quot;Calibri&quot;,sans-serif"><span style="color:black"> value of 0.347 mg/mL.</span></span></span><span lang="TR" style="font-size:11.0pt"><span style="font-family:&quot;Calibri&quot;,sans-serif"><span style="color:black"> The prepared films were evaluated for thickness, solubility, oxygen permeability, water vapor permeability, and mechanical properties. Zein films exhibited the highest values for solubility, elongation at break, oxygen permeability, and tensile strength, whereas the lowest values were recorded for the Zein + 5% Fucoidan + 2.5% TiO</span></span></span><span lang="TR" style="font-size:11.0pt"><span style="font-family:&quot;Cambria Math&quot;,serif"><span style="color:black">₂</span></span></span><span lang="TR" style="font-size:11.0pt"><span style="font-family:&quot;Calibri&quot;,sans-serif"><span style="color:black"> nanocomposite (<i>p</i>&le;0.05</span></span></span><span lang="TR" style="font-size:11.0pt"><span style="font-family:&quot;Calibri&quot;,sans-serif"><span style="color:black">). FTIR, DSC, and XRD results confirmed interplays between TiO</span></span></span><span lang="TR" style="font-size:11.0pt"><span style="font-family:&quot;Cambria Math&quot;,serif"><span style="color:black">₂</span></span></span><span lang="TR" style="font-size:11.0pt"><span style="font-family:&quot;Calibri&quot;,sans-serif"><span style="color:black"> nanoparticles and the carbonyl and carboxyl functional groups within the nanocomposite matrix. The study acknowledges that further investigations are needed to assess long-term storage stability and performance in real food systems. This study introduces a novel bioactive nanocomposite film with enhanced barrier and mechanical properties, distinguishing it from conventional biodegradable films. The Zein + 5% Fucoidan + 2.5% TiO</span></span></span><span lang="TR" style="font-size:11.0pt"><span style="font-family:&quot;Cambria Math&quot;,serif"><span style="color:black">₂</span></span></span><span lang="TR" style="font-size:11.0pt"><span style="font-family:&quot;Calibri&quot;,sans-serif"><span style="color:black"> nanocomposite was identified to have the optimal formulation, demonstrating superior performance and having potential for application in food preservation.</span></span></span> Titanium dioxide, Sargassum tenerrimum, Nanocomposite, Fucoidan 467 494 http://jifro.ir/browse.php?a_code=A-10-5300-1&slc_lang=en&sid=1 S. Chahkhoozadeh Dr.chahkhoozadeh@yahoo.com 100319475328460058820 100319475328460058820 No Department of Science and Research, Islamic Azad University, Qeshm Branch, Qeshm, Iran A. Akhondzadeh Basti aakhond@ut.ac.ir 100319475328460058821 100319475328460058821 No Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran M. Tala maryam_talla4349@iau.ac.ir 100319475328460058822 100319475328460058822 Yes Department of Fisheries, Islamic Azad University, Qeshm Branch, Qeshm, Iran A.A. Anvar saaa4824@gmail.com 100319475328460058823 100319475328460058823 No Department of Food Hygiene, Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Iran A. Khanjari Khanjari@ut.ac.ir 100319475328460058824 100319475328460058824 No Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran