1 1562-2916 Iranian Fisheries Research Organization 3841 Bio-functions of carvacrol-supplemented feeds on lipopolysaccharide-induced rainbow trout (Oncorhynchus mykiss Walbaum, 1792) Kucukgul A. b Kucukgul A. c Gulsafak İ. d b Tunceli University, Fisheries Faculty, TR62000, Tunceli, Turkey c Mustafa Kemal University, Veterinary Faculty, TR31000, Hatay, Turkey d Tunceli University, Fisheries Faculty, TR62000, Tunceli, Turkey 1 4 2019 18 2 205 214 17 03 2019 17 03 2019 To investigate the effects of carvacrol supplementation in rainbow trout induced by lipopolysaccharide (LPS), relative gene expressions levels of some pro-inflammatory cytokines and apoptosis markers in kidney and liver were measured. Fish with a mean weight of 44.71±1.33 g were studied using four different treatments with three replicates each. Inflammation and apoptosis were performed using LPS of Escherichia coli (25 µg ml-1) except in the control group and only carvacrol (100 µg ml-1 diet) containing diets were fed to this group (CAR). The last group was the infected fish fed carvacrol supplemented diet (+CAR). Kidney and liver tissues were removed 3 days after to determine the levels of interleukin-1β (IL-1β), interferon gamma (INF-γ), caspase 3 (Cas 3), caspase 8 (Cas 8) using Real-Time PCR analyses. IL-1β expressions of both kidney and liver was significantly decreased (12.9 and 2.14 fold, respectively) in LPS treated cells (p<0.05). While IFN-γ expression was up regulated in kidney, it had down regulation in liver. LPS decreased both Cas 3 and 8 expressions in kidney but increased in liver. +CAR increased expressions of IL-1β and IFN-γ compared with the control (up to 2-3 fold) in all tissues except caspase gene expressions that were similar in the control. In all tissues, IL-1β and IFN-γ expressions increased in +CAR group, expect IFN-γ in the kidney. However, Cas 3 and 8 expressions including apoptosis was induced by up-down regulations in all tissues compared to LPS-injected fish. The results showed that carvacrol had pro-inflammatory and apoptotic effects especially on liver tissue of LPS-induced inflammation model of Oncorhynchus mykiss.  
3842 Effect of using various amounts of patin (Pangasianodon hypophthalmus) fish oil on physical and chemical properties of moisturizing hand cream Shabanikakroodi S. e Christianus A. f Tan C.P. g Shabanikakroodi S. h Ehteshami F. i e Institute of Bioscience and Biotechnology, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia. f Institute of Bioscience and Biotechnology, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia. g Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia. h Faculty of Engineering, University of Tehran, 13145-1318 Tehran, Iran. i Iranian Fisheries Science Research Organization (IFSRO), Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran. 1 4 2019 18 2 215 223 17 03 2019 17 03 2019 Hand cream is used for rehydrating and improving skin properties. The skin is the first point of contact with the environment. Thus having healthy skin with proper barrier properties is quite important. The beneficial effects of fish oil on skin make it a favorable ingredient to be used in hand care products. In the present study, the efficacy and stability of various hand cream formulations using 0, 1.0, 2.5 and 5.0% of fish oil were investigated and compared. The appearance, odor, texture, and pH of formulations containing 1 and 2.5% of fish oil remained stable in very good qualities during the storage, whereas the related values to the appearance and texture of formulation containing 5.0% of fish oil at the end of 6 months of storage at 45°C were significantly (p<0.05) lower than other formulations and previous evaluations of this formulation. Weight loss was not observed in all formulations under study.  3843 Quality enhancement in refrigerated tiger tooth croaker (Otolithes ruber) fillets using chitosan coating containing green tea extract Khodanazary A. j Salati A.P. k Bohlouli S. l Mohammadi M. m Sadeghi E. n j Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran. k Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran. l Department of Veterinary Medicine, Faculty of Agriculture, Kermanshah Branch, Islamic Azad University, Kermanshah Iran. m Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran. n Research Center for Environmental Determinants of Health (RCEDH), Kermanshah University of medical Sciences, Kermanshah, Iran. 1 4 2019 18 2 224 241 17 03 2019 17 03 2019 The effects of chitosan (2%) coating combined with green tea extract (GTE) (1%) on the quality of tiger-tooth croaker (Otolithes ruber) fillets including chemical, microbiological, texture, color and sensory properties during 16 days of refrigerated storage was investigated. The color changes were significantly retared, and the texture parameteres and sensory scores were significantly improved in tiger- tooth croaker coated by chitosan, GTE and chitosan combined with GTE, compared with the control. The coincidental lowered rate of increase total volatile base (TVB) content, thiobarbitoric acid (TBA), peroxide value (POV) and free fatty acids (FFA) were obtained in tiger-tooth croaker coated chitosan+GTE. Chitosan in combination with GTE had higher inhibition on microbial growth and yielded the tigertooth croaker with higher likeness, comapared with the other treatments. Therefore, fish coated with chitosan+GTE had the lowest losses in quality during refrigerated storage. 3844 Blood biochemical changes in common carp (Cyprinus carpio) upon co-exposure to titanium dioxide nanoparticles and paraquat Banaee M. o Tahery S. p Nematdoost Haghi B. Shahafve Sh. Vaziriyan M. o Department of Aquaculture, Faculty of Natural Resources and Environment, Behbahan Khatam Al-anbia University of Technology, Khuzestan Province, Iran p Department of Aquaculture, Faculty of Natural Resources and Environment, Behbahan Khatam Al-anbia University of Technology, Khuzestan Province, Iran Department of Aquaculture, Faculty of Natural Resources and Environment, Behbahan Khatam Al-anbia University of Technology, Khuzestan Province, Iran Department of Aquaculture, Faculty of Natural Resources and Environment, Behbahan Khatam Al-anbia University of Technology, Khuzestan Province, Iran Department of Aquaculture, Faculty of Natural Resources and Environment, Behbahan Khatam Al-anbia University of Technology, Khuzestan Province, Iran 1 4 2019 18 2 242 255 17 03 2019 17 03 2019 Research on eliminating organic pollutants in water by using heterogeneous photocatalysts such as nano-TiO2 abound. However, the question is whether metabolites, resulting from optical dispersion of environmental pollutants, are still toxic to aquatic organisms. Therefore, this study was designed to evaluate the effect of a co-exposure to paraquat and TiO2-NPs on blood biochemical indices of common carp. Fish were exposed to 0.2 and 0.4 mg L-1 paraquat with 0.125 mg L-1 TiO2-NPs for 21 days under different light conditions, including natural photoperiod (16 L 8h-1 D) and complete darkness. No significant alterations in the Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Lactate dehydrogenase (LDH), Alkaline phosphatase (ALP) and Creatine phosphokinase (CPK), activities and total protein, albumin, globulin, glucose, cholesterol and triglyceride levels were observed in fish exposed to TiO2-NPs and 0.2 mg L-1 paraquat under normal lighting conditions. However, a significant change in blood biochemical indices in fish exposed to TiO2-NPs with 0.4 mg L-1 paraquat (16 L 8h-1 D) and in fish exposed to TiO2-NPs and paraquat (under darkness). A significant decrease in the activity of Gamma-glutamyltransferase (GGT) and a significant increase in creatinine level were observed in all groups which were exposed to TiO2-NPs and paraquat. The results of this study indicate that using 0.125 mg L-1 nano-TiO2 in order to remove paraquat (0.2 mg L-1) under lighting conditions can minimize the adverse effects of paraquat and its metabolites on blood biochemical indices of fish. So, using nano-TiO2 (0.125 mg L-1) to remove paraquat under lighting conditions can significantly reduce its toxic effects. 3845 Shells of Bufonaria echinata as biomonitoring materials of heavy metals (Cd, Ni and Pb) pollution in the Persian Gulf: with emphasis on the annual growth sections Pourang N. Bahrami A. Nasrolahzadeh Saravi H. Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension, Organization, Tehran, Iran. Bandar Abbas Branch, Islamic Azad University, Bandar Abbas Branch, Iran. Caspian Sea Ecology Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Sari, Iran. 1 4 2019 18 2 256 271 17 03 2019 17 03 2019 Concentrations of heavy metals (Cd, Ni and Pb) were determined in soft and hard tissues (three separated shell sections) of gastropod Bufonaria echinata as well as surficial sediments collected in October 2015 from two sampling sites located in the sub-littoral zone of Qeshm Island, Persian Gulf. There were significant differences between the sampling sites for concentrations of all the three elements in the shells and sediments. But in terms of the soft tissues, in the case of Ni and Pb significant differences between the sites could be observed. In all the cases, higher levels were observed in the samples from Suza site, which may be mainly due to the proximity of this site to the relevant anthropogenic sources. Comparison of the gained data from this study with the other relevant researches shows that in most cases the levels of the elements in the soft tissues and shells either fell within the range for other world areas or were lower. The observed increasing trends of metals accumulation in the shell sections (from older to younger sections) could be mainly attributed to the gradual increase of relevant anthropogenic pollutants in the study area, especially in Suza pier, during the recent years. Generally, it can be concluded that the shells of B.echinata could be possibly employed as a biomonitoring tool for historic metals contamination in northeastern part of the Persian Gulf. 3846 Expression of estrogenic response genes to different concentration of 17ß-estradiol in male mosquitofish (Gambusia yucatana) Rendón von Osten J. Aguayo-Dionet G. Dzul-Caamal R. Lara-Flores M. Instituto de Ecología, Pesquerías y Oceanografía del Golfo de México, Universidad Autónoma de Campeche, San Francisco de Campeche, Campeche, México Instituto de Ecología, Pesquerías y Oceanografía del Golfo de México, Universidad Autónoma de Campeche, San Francisco de Campeche, Campeche, México Instituto de Ecología, Pesquerías y Oceanografía del Golfo de México, Universidad Autónoma de Campeche, San Francisco de Campeche, Campeche, México Instituto de Ecología, Pesquerías y Oceanografía del Golfo de México, Universidad Autónoma de Campeche, San Francisco de Campeche, Campeche, México 1 4 2019 18 2 272 282 17 03 2019 17 03 2019 The estrogenic effects of endocrine disrupting compounds in fish are not reversible and can reduce populations. Sensitive methods such as Q-PCR, Western blot, microarrays, have been used to determine changes in gene expression and this predict the effects before they become irreversible. The present study was designed to detect the expression of the estrogen receptors vitellogenin and pregnane X indicates that they are potentially useful molecular markers for detecting the presence of endocrine disrupting compounds in the environment. The results demonstrate that the exposure to the hormone 17ß-estradiol induced the expression of estrogenic response genes in male mosquito fish (G. yucatana) and this is related to the alterations to the endocrine system caused by the hormone. 3847 Effect of the probiotic, Lactobacillus plantarum on growth performance and haematological indices of rainbow trout (Oncorhynchus mykiss) immunized with bivalent streptococcosis / lactococcosis vaccine Soltani M. Kane A. Taheri-Mirghaed A. Pakzad K. Hosseini-Shekarabi P. Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Departmet of Fisheries Science, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran. 1 4 2019 18 2 283 295 17 03 2019 17 03 2019 The present study evaluated the effect of Lactobacillus plantarum as a probiotic on the growth performance and haematological parameters of juvenile rainbow trout (Oncorhynchus mykiss) weighing 29.6±1.8 g immunized with streptococcus/lactococcus bivalent vaccine for 60 days at 16±1.5ºC. Fish were randomly allocated to 4 equal groups: probiotic (g kg-1 feed equal to 108 cells g-1) supplemented diet group (group P), vaccinated fish fed with a normal diet (group V), vaccinated fish fed with probiotic (group P+V) and unvaccinated fish fed normal diet (group C). Results showed that weight gain, final weight, condition factor and thermal-unit growth coefficient were improved in P+V group compared with other groups but was significantly different from groups V and C (p<0.05). Also, feed conversion ratio in the P+V group was lower than other trails (p<0.05). In addition, white blood cell count (WBC) in groups V and P+V were significantly higher than in both P and C groups (p<0.05). Also, PCV was significantly increased (p<0.05) in both P and P+V groups compared to group V and the control group. No significant difference (p>0.05) was found in RBC, Hb, MCH, MCHC and MCV levels among the different experimental groups. These data show that application of L. plantarum could be a benefit of vaccinated trout, enhancing fish growth and haematological parameters 3848 Estimation of Otolithes ruber stock with virtual population analysis in the Northwest area of the Persian Gulf Eskandari Gh. Koochaknejad E. Hashemi S.A. Southern Iran Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran Iranian National Institute for Oceanography and Atmospheric Science, Chabahar, Iran Offshore Fisheries Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Chabahar, Iran 1 4 2019 18 2 296 306 17 03 2019 17 03 2019 Stock assessment of Otolithes ruber was carried out using Virtual Population Analysis (VPA) method in the coastal waters of Northwest area of the Persian Gulf. Catch and length frequency data during 2002-2012 were converted to age frequency. The maximum of stock number and biomass were observed in age group 1 and 2, respectively. The minimum stock number and biomass were observed for age group 6. The maximum and minimum catch numbers were in age groups 2 and 6, respectively. The maximum and minimum of weight were in age group 3 and 1, respectively. The maximum and minimum of instantaneous fishing mortality were in age groups 4 and 1, respectively. The maximum fishing mortality was estimated in 2010-2011 and minimum value was in 2002-2003 and 2008-2009. During 2011-2012 the stock number and biomass were estimated 29558 (*103) specimens, 7302 tones and the catch number and weight were calculated 7562 (*103), 2364 tones and fishing mortality was obtained 0.47 per year. The calculated parameters showed over-exploitation in age-groups 3 and 4. To preserve stocks and to guarantee sustainable exploitation, the prevention of increasing fishing efforts and the control of fishing activities are crucial.   3849 Effects of sumithion on growth and production of phytoplankton and zooplankton in aquaculture ponds Hossain M.I. Rahman M.S. Amin A.K.M.R. Ahmed S.I. Shahjahan M. Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. Department of Fisheries Biology and Genetics, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh. Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. 1 4 2019 18 2 307 318 17 03 2019 17 03 2019 Sumithion is an organophosphorous pesticide widely used to control tiger bugs in fish larval rearing pond. The present study was aimed to investigate the effects of sumithion on plankton population abundance in aquaculture pond. The experiment was carried out with three treatments, i.e. ponds with no sumithion (T1), ponds with 1.0 ppm sumithion (T2) and those with 2.0 ppm sumithion (T3). The water quality parameters, such as temperature, pH, dissolved oxygen and total alkalinity were almost unchanged throughout the study period whereas transparency, NO3-N and PO4-P values declined with an increase in sumithion concentrations but differences were not significant (p<0.05). No distinct changes were observed in population densities of phytoplankton (x cells L-1). On the other hand, the zooplankton population densities (x Ind L-1) significantly (p<0.05) decreased with toxicity of sumithion after 30 days up to the end of experimental period in both T2 and T3 compared to the control group (T1). The ranges of pH, organic carbon (%), available phosphorus (ppm) and total nitrogen (%) of pond bottom-sediment did not differ irrespective of the treatments. This study demonstrated that sumithion has adverse effects on zooplankton which may influence the production in aquaculture pond.  3850 Evaluation of some feeding habits of Rastrelliger kanagurta (Cuvier, 1817) in the Persian Gulf (Hormozgan Province) Daghooghi B. Kaymaram F. Vosoughi A. Valinassab T. Moradi M. Islamic Azad University. Sciences and Research Branch. Tehran, Iran. Iranian Fisheries Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Iran. Islamic Azad University. Sciences and Research Branch. Tehran, Iran. Iranian Fisheries Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Iran. Iranian National Oceanography Center. Tehran, Iran. 1 4 2019 18 2 319 331 17 03 2019 17 03 2019 The Indian mackerel (Rastrelliger kanagurta, Cuvier, 1817) is one of the commercial small pelagic fish in the Persian Gulf and Oman Sea. In the current research, feed preference index (FP), fullness index (FI), and stomach contents of Rastrelliger kanagurta were evaluated to assess the quantity and type of feed habits. A total of 573 specimens were collected randomly from Bandar - Abbas and Qeshm Island fishing sites from November 2011 to October 2012. The minimum and maximum total lengths were 13.9 and 35.5cm, respectively with the highest frequency in length group of 24-26cm. Results showed that 21.3% of stomachs were full, 44.5% were semi-full and 34.2% were empty. Planktons and fish were the main and random feed items of this species respectively. Fullness index and Vacuity index for this species were calculated 21.3 and 34.2, respectively. Stomach contents analysis revealed that phytoplankton (66%) and zooplankton (34%) form the main feed of Rastrelliger kanagurta. Among the phytoplankton, Bacillariophyceae (86%) was the dominant feed followed by Cyanophyceae (8%) and Dynophyceae (6%). Copepods (88%) were dominant zooplanktonic feed items. Encrasicholina punctifer was the only bony fish observed. Maximum and minimum of GaSI index were estimated 2.57 and 1.12 in October and February, respectively.  Condition factor and relative guts length were calculated 1.76 and 2.38, respectively. The results indicated that this fish is a relatively frugal species which consumes plankton as the main feed.   3851 Diversity and distribution of larval and juvenile fish in nearshore waters of the Southeastern Caspian Sea and Gorgan Bay Shahlapour S. Afraei Bandpei M.A. Rabbaniha M. Pourang N. Nasrollahzadeh H. Caspian Sea Ecology Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Sari, Iran Caspian Sea Ecology Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Sari, Iran Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran Caspian Sea Ecology Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Sari, Iran 1 4 2019 18 2 332 348 17 03 2019 17 03 2019 Occurrence and distribution of larval and juvenile fish was investigated in nearshore waters of the southeastern Caspian Sea including the Gorgan Bay. Samples were collected during seven surveys using a fine mesh beach-seine in five sampling sites from July 2014 to June 2015. A total of 15500 individuals mostly juvenile were captured, representing 18 species from 8 families. Most juveniles belonged to Liza saliens, Atherina caspia, Neogobius pallasi and Neogobius melanostomus.  The juveniles of Alburnus chalcoides, Vimba persa, Syngnathus caspius and gobies Neogobius caspius and Ponticola syrman occurred in low number (relative abundance 0.1% - 9.3%). Highest number of juveniles were caught in summer season. The occurrence of larval fish in coastal waters of southern Caspian was recorded for the first time (Atherina caspia with minimum length of 12 mm in Caspian and 9.5 mm in Gorgan Bay). In spring, early juveniles of mullet, Liza aurata, increased in the Caspian sites but they moved up to deeper waters by the end of the season. Based on results, the density of juvenile fish (specially Liza saliens) in Gorgan Bay was several times more than Caspian sites so it could be considered an important nursery ground in the region 3852 Virulence of Aeromonas hydrophila in Siamese fighting fish (Betta splendens) and the bacterium susceptibility to some herbal plants Kanchan C. Imjai P. Kanchan N. Panchai K. Hatai K. Program in Aquaculture Technology, Faculty of Agriculture Technology, Rajabhat Maha Sarakham University, 44000, Thailand Program in Aquaculture Technology, Faculty of Agriculture Technology, Rajabhat Maha Sarakham University, 44000, Thailand Animal Production Technology Program, Faculty of Agro-Industrial Technology, Kalasin University, 46000, Thailand Biology Program, Faculty of Science and Technology, Nakhon Ratchasima Rajabhat University, 30000, Thailand Borneo Marine Research Institute, University Malaysia Sabah, Malaysia, 88400 Kota Kinabalu, Sabah, Malaysia 1 4 2019 18 2 349 354 17 03 2019 17 03 2019 Aeromonas hydrophila was isolated from diseased Siamese fighting fish Betta splendens Regans.  The virulence of A. hydrophiala to Siamese fighting fish was conducted by intraperitoneal injection of two doses of bacterial suspensions with 7.5×107 and 7.5×105 cfu per 0.05 ml. The cumulative mortality was observed in high and low dose groups which showed 98.33 and 20 percent, respectively. Moreover, five medicinal plants extracts namely Centella asiatica, Morinda citrifolia, Melissa officinalis, Piper sarmentosum and Terminalia catappa were determined for antimicrobial activities by broth dilution method. Terminalia catappa extract showed highest antimicrobial effect of MIC and MBC (25 and 12.5 mg mL-1), followed by M. officinalis and P. sarmentosum with the same values of MIC and MBC (12.5 and 25 mg mL-1).  Finally, Centella asiatica and Morinda citrifolia were similarly MIC and MBC (50 and 100 mg mL-1).  It was concluded that five medicinal plants can use to inhibit and kill A. hydrophila in fish.    3853 Identification and investigation of species diversity and richness of the Gastropoda in intertidal zone of Bushehr Port coastal area (the Persian Gulf waters) Pazira A.R. Salehi H. Obeidi R. Department of Natural Resources- Reproduction and Culture of Aquatics, Bushehr Branch, Islamic Azad University, Bushehr, Iran. Young Researchers and Elite Club, Bushehr Branch, Islamic Azad University, Bushehr, Iran. Young Researchers and Elite Club, Bushehr Branch, Islamic Azad University, Bushehr, Iran. 1 4 2019 18 2 355 370 17 03 2019 17 03 2019 This study aims to identify and determine species diversity and richness of the Gastropods of the intertidal zone of Bushehr seaport coastal area and assess the ecological status of this area using Welch index. Sampling was carried out at 6 stations in Bushehr seaport in the winter and summer seasons during 2013-2014. Bed sediment sampling was done three times in each station and Gastropod samples were collected using Quadrate (25×25 cm), and were then preserved using 4 % buffered formalin. The preserved samples were transported to the laboratory and were identified and counted by using valid identification keys. Furthermore, physicochemical properties of water including temperature, pH, dissolved oxygen (DO) and salinity were also measured in each station. According to the results obtained from this study, there were totally 14 species identified from 12 families belonging to 7 orders. The most abundantly identified species in the studied stations belonged to Planaxis sulctus, Cerithidea cingulate, and Anachis misera. In this study species diversity was determined by using Shannon-Weiner index, dominant species were investigated using Simpson index and species richness was calculated using Margalef index. The results of Shannon and Margalef indexes showed significant differences between the sampled stations and seasons (p˂0.05). Moreover, the results of Simpson index showed no significant differences between the sampled stations and seasons (p<0.05). Results of Welch index showed that based on the mentioned indexes in the sampled stations the status of the water quality was classified as average and rather low/weak pollution.  3854 Short communication:Effect of salt and alkaline on the physicochemical properties of the protein isolates extracted from lanternfish (Benthosema pterotum) Oliyaei N. Moosavi-Nasab M. Ghorbani M. Seafood Processing Research Group, Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Iran Seafood Processing Research Group, Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Iran Department of Food Science and Technology, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran 1 4 2019 18 2 371 385 17 03 2019 17 03 2019 Food proteins have long been recognized for their nutritional and functional properties. The nutritional properties of proteins are associated with their amino acid content. On the other hand, the functional properties of proteins relate to their contribution to the physiochemical and sensory properties of foods (Sila and Bougatef, 2016). Marine organisms contain proteins with high quantities of essential amino acids which can provide the high quality proteins for the human consumption (Cordova Murueta et al., 2007). Fish muscle proteins are categorized in to three groups based on their solubility in aqueous solutions. Myofibrillar protein (soluble in high salt concentration), sarcoplasmic protein (soluble in water and weak salt solutions) and stromal protein (insoluble proteins) (Karlsdottir, 2009). 3855 Short communication:In vitro antibacterial effect of ginger (Zingiber officinale) essential oil against fish pathogenic bacteria isolated from farmed olive flounder (Paralichthys olivaceus) in Korea Hossain S. De Silva De Silva Wimalasena S.H.M.P. Pathirana Pathirana Heo G.J. Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, Korea Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, Korea Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, Korea Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, Korea Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644, Korea 1 4 2019 18 2 386 394 17 03 2019 17 03 2019 World population growth and food demand have been two major elements leading to the expansion of animal and fish production. Recently, many efforts advocated the extension of intensive fish farming to optimize the need for animal protein (Hussein et al., 2013). In Korea, aquaculture has made rapid development in a short period of time, and many fish farms have been established in order to fulfill high consumer demand (Oh et al., 2006). Meanwhile, olive flounder (Paralichthys olivaceus) is one of the popular marine fishes cultured in Korea, which accounts for approximately 50% of the annual fish production (Park et al., 2012).