Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1391
4
1
gregorian
2012
7
1
11
3
online
1
fulltext
en
Experimentally pathogenesis of Aeromonas hydrophila in freshwater Crayfish (Astacus leptodactylus) in iran
fish disease
fish disease
پژوهشي
Orginal research papers
<font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: 150% unicode-bidi: embed direction: ltr text-justify: kashida text-kashida: 0%"><font size="3"><font face="Times New Roman"><span style="mso-bidi-language: FA">In this research the affect of <i>A. hydrophila</i> on pathogenesis of Crayfish
<i>A.</i> <i>leptodactylus</i> was carried out<span style="color: black">.</span>
This study was designed in three groups as treatments </span>and one group as
control in triplicate with 20 Crayfish in each glass aquarium.</font></font><span style="mso-bidi-language: FA"><font face="Times New Roman" size="3"> The treatments have exposed to <i>A. hydrophila</i>.
The concentrations of the bacteria in treatments were </font><span style="top: 5pt position: relative mso-text-raise: -5.0pt"><v:shapetype id="_x0000_t75" stroked="f" filled="f" path="m@4@5l@4@11@9@11@9@5xe" o:preferrelative="t" o:spt="75" coordsize="21600,21600"><font size="3"><font face="Times New Roman">
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<v:imagedata src="file:///C:UsersSHBF9D~1.KAKAppDataLocalTempmsohtmlclip1 1clip_image001.wmz"> </v:imagedata></font></font></v:shape></span><font face="Times New Roman" size="3">, and</font><span style="top: 3pt position: relative mso-text-raise: -3.0pt"><v:shape id="_x0000_i1026" style="width: 33pt height: 15.75pt" type="#_x0000_t75"><font size="3"><font face="Times New Roman">
<v:imagedata src="file:///C:UsersSHBF9D~1.KAKAppDataLocalTempmsohtmlclip1 1clip_image003.wmz"> </v:imagedata></font></font></v:shape></span><font face="Times New Roman"><font size="3"><span style="mso-spacerun: yes"> </span>CFUmL</font><sup><font size="2">-1</font></sup><font size="3"> respectively.
The untreated control group was disinfected by oxytetracyclin at concentration of
100 ppm for 24 hours. The haemolymph samples were withdrawn for measuring of
THC and TPC within interval hours (2, 6, 12, 24, 48, 96, 144, 240 and 336). The
Crayfish samples were then fixed in Davidson fixative. The results showed that
the differences of THC value between treatment 4 (95.33±23.16) and control
(1.13±.45) were significant (<i>P<0.05</i>) as well as control and
treatments 3 (35.33±16.16) and 4 (95.33±23.16) during 2 initial hours. In case
of THC the significant difference (<i>P<0.05</i>) was observed between
control group (72.33±15.04) and treatment 3 (13.13±5.85) after 48 hours. During
240 hours, also the difference of THC value between control group (80.33±24.94)
with treatment 2 (14.67±3.51) was significant (<i>P<0.05</i>). The finding
of TPP value showed that there was no significant difference between control
group and treatments in during mentioned times (<i>P<0.05</i>). The result
of histopathology in heaptopancreas and gill sections showed that hemocyte
aggregation and necrosis within pyknosis of nucleus. In lower concentrations of
bacteria no pathological changes of heart were observed. In digestive tract no
changes were appeared in treatments, but in concentration of </font></font><span style="top: 3pt position: relative mso-text-raise: -3.0pt"></span><font face="Times New Roman"><font size="3"><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: FA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">3×10<sup>8</sup> </span>CFU ml</font><sup><font size="2">-1 </font></sup><font size="3">a low aggregation of hemocytes was
revealed. <o:p /></font></font></span></p><font face="Times New Roman" size="3"> </font>
Astacus leptodactylus, Aeromonas hydrophilia, Total Hemocyte Count, Total Protein Concentration, Histopathology
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656
http://jifro.ir/browse.php?a_code=A-10-1-186&slc_lang=en&sid=1
A.
SamCookiyaei
Drsam.arash@gmail.com
10031947532846004020
10031947532846004020
Yes
M.
Afsharnasab
10031947532846004021
10031947532846004021
No
V.
Razavilar
10031947532846004022
10031947532846004022
No
A. A.
Motalebi
10031947532846004023
10031947532846004023
No
S.
Kakoolaki
10031947532846004024
10031947532846004024
No
Y.
Asadpor
10031947532846004025
10031947532846004025
No
M. Y.
Yahyazade
10031947532846004026
10031947532846004026
No
A.
Nekuie Fard
10031947532846004027
10031947532846004027
No