en Stock assessment Stock assessment پژوهشي Orginal research papers <p style="text-align:justify"><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">This study successfully determined the chromosome numbers and karyotypes of two marine fish species from separate families using the short-term culture method (PB-MAX&trade; application). The culture incubation periods varied for each species. Kidney tissues from the fish samples were treated with PB-MAX&trade; for either 2.5 or 4 h. Results showed that diploid chromosome numbers, karyotypes, and arm numbers differ by species: Red Mullet, <i>Mullus barbatus</i>, has 2n &ndash; 44, with 3 pairs of metacentric, 7 pairs of submetacentric, 7 pairs of subtelocentric, and 5 pairs of acrocentric chromosomes (NF=64). Round Goby, <i>Neogobius melanostomus</i>, has 2n &ndash; 46, consisting of 46 pairs of acrocentric chromosomes (NF=46). C&ndash; and NOR&ndash;positive results were achieved through constitutive heterochromatin banding and silver nitrate staining for both species. The primary objective of this research is to demonstrate the applicability of short-term culture in producing successful cytogenetic results in marine fish. This study will support future research and facilitate chromosome studies on marine fish, making the process quick and practical.</span></span></span></p> <div> <div> <div class="msocomtxt" id="_com_1" language="JavaScript" on=""><illegal tag=""></illegal></div> </div> </div> Marine fishes, Mullus barbatus, Neogobius melanostomus, Chromosome, Short-term culture 1477 1493 http://jifro.ir/browse.php?a_code=A-10-2362-1&slc_lang=en&sid=1 S. Saygun ssaygun@odu.edu.tr 100319475328460058204 100319475328460058204 Yes Ordu University, Faculty of Marine Science, Fishery Technology Engineering, Department of Aquaculture, Fatsa, Ordu, Turkey M. Gundogan murat.gundogan@outlook.com 100319475328460058205 100319475328460058205 No Ordu University, Institute of Science, Ordu, Turkey