en aquaculture aquaculture كاربردي Orginal research papers <span lang="EN" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">A biological filter (biofilter) is a technique that aims to degrade nutrients that are wasted in water bodies by utilizing living organisms such as plants and shellfish. </span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">The study aims to determine the potential of duckweeds (<i>Lemna minor</i>)-freshwater mussel (<i>Anodonta cygnea</i>) integrated biofilters in utilizing nutrients from wastewater from rearing rainbow trout (<i>Oncorhynchus mykiss</i>) juveniles. This study used the initial weight of 25&plusmn;2.0g of rainbow trout juveniles, freshwater mussels (<i>A. cygnea</i>) with an initial weight of 58&plusmn;2.0 g, and duckweeds (<i>L. minor)</i> with two different biomass weights (treatment 1: 270 g and treatment 2: 360 g) were used in the flow-through system. The water temperature was around 13-14^oC during the experimental periods. Using duckweeds as a biofilter with two different stocking densities significantly affected (<i>p</i><0.05) the pH, DO, nitrate, and total phosphorus values in the treatment tanks. This study&#39;s results also revealed that using duckweed-freshwater mussels integrated biofilters could minimize the concentration of ammonium and total phosphorus in wastewater from rainbow trout juvenile rearing tanks.</span></span></span> Lemna minor, Anodonta cygnea, Oncorhynchus mykiss, Living Biofilter, Aquaculture wastewater 371 388 http://jifro.ir/browse.php?a_code=A-10-4792-1&slc_lang=en&sid=1 M.H. Azhar hanifazhar1811@gmail.com 100319475328460051342 100319475328460051342 Yes Department of Aquaculture, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya, Indonesia D. Memiş mdevrim@istanbul.edu.tr 100319475328460051343 100319475328460051343 No Department of Aquaculture and Fish Diseases, Faculty of Aquatic Sciences, Istanbul University, Onaltı Mart Şehitleri Avenue, No. 2, 34134, Vezneciler, Fatih, Istanbul, Turkey