en aquaculture aquaculture پژوهشي Orginal research papers <span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">The present study</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black"> investigated the effects of water temperature and culture duration on <i>Silurus asotus</i> or <i>Anguilla japonica</i> growth</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black"> performance</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black"> and bacterial community in a bio</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">floc technology (BFT)</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">-based system. </span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">A temperature-dependent higher growth performance was observed in </span></span></span><i><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">S. asotus</span></span></span></i><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black"> or <i>A. japonica</i> with </span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">the highest growth rate at 30</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">℃</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black"> culture temperature. In 25</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">℃</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">-BFT tanks, the bacterial diversity achieved the highest value before rearing the fish, while the highest bacterial diversity was observed in 30</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">℃</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">-BFT tanks after rearing eel or catfish. The </span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">bacterial communities were differentially clustered depending on the temperature, culture duration, and fish species. Proteobacteria and bacteroidetes were the most dominant phyla</span></span></span> <span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">and 45 predominant genera were identified.</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black"> Among the predominant genera, <i>Cetobacterium</i> was the most abundant which accounts for 16-38% in the BFT tanks of catfish with different water temperatures. <i>Nannocystis</i> (22.3%), <i>Cetobacterium </i>(10.2%), and <i>Bacillus </i>(13.3%) were observed the most abundant in the 20</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">℃</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">, 25</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">℃</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">, or 30</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">℃</span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black"> maintained BFT tanks of eel, respectively. </span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">Notably, </span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">the correlation analysis demonstrated </span></span></span><i><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">Devosia</span></span></span></i><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black"> abundance could affect catfish growth positively, while </span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">the </span></span></span><i><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">Flavobacterium</span></span></span></i><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black"> abundance could affect eel growth</span></span></span> <span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">negatively. Additionally, <i>Exiguobacterium acetylicum</i> SK4913 exhibited the most probiotic potential among the 27 isolates from </span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">all the </span></span></span><span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">BFT tanks considering its multi-enzymatic activities. In summary, the culture temperature at the biofloc-preparation stage and fish growth stage should be maintained at 20℃ and 25-30℃, respectively.</span></span></span><br> <span style="font-size:10pt"><span style="line-height:200%"><span style="word-break:normal"><span gothic="" malgun="" style="font-family:"><span lang="EN-US" style="font-size:12.0pt"><span style="line-height:200%"><span style="font-family:"TimesNewRoman","serif""></span></span></span></span></span></span></span> Biofloc, Microbiota, Next-generation sequencing, Probiotic, BFT 795 818 http://jifro.ir/browse.php?a_code=A-10-4688-1&slc_lang=en&sid=1 Y.G. Kim ykkim@konkuk.ac.kr 100319475328460056825 100319475328460056825 No Department of Animal Science and Technology, Konkuk University K.M. Niu niukaimin@naver.com 100319475328460056826 100319475328460056826 No Institute of Biological Resources, Jiangxi Academy of Sciences J.A. Hwang hjuae1031@korea.kr 100319475328460056827 100319475328460056827 No Advanced Aquaculture Research Center, National Institute of Fisheries Science H.S. Kim kimk2k@korea.kr 100319475328460056828 100319475328460056828 No Advanced Aquaculture Research Center, National Institute of Fisheries Science J.S. Park pjs5420939@gmail.com 100319475328460056829 100319475328460056829 No Advanced Aquaculture Research Center, National Institute of Fisheries Science J.H. Lee jhlee7124@korea.kr 100319475328460056830 100319475328460056830 No Fish Genetics and Breeding Research Center, National Institute of Fisheries Science G.W. Ga rkrjsgnl@konkuk.ac.kr 100319475328460056831 100319475328460056831 No Department of Animal Science and Technology, Konkuk University S.W. Jeon wjs488678@naver.com 100319475328460056832 100319475328460056832 No Department of Animal Science and Technology, Konkuk University A.R. Lee 100319475328460056833 100319475328460056833 No Animal Resources Research Center, Konkuk University, Seoul 05029, Republic of Korea S.K. Kim sookikim@konkuk.ac.kr 100319475328460056834 100319475328460056834 Yes Department of Animal Science and Technology, Konkuk University