Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1391
1
1
gregorian
2012
4
1
11
2
online
1
fulltext
en
Anticancer effect of Dunaliella salina under stress and normal conditions against skin carcinoma cell line A431 in vitro
Biology & physiology
Biology & physiology
پژوهشي
Orginal research papers
<font face="Times New Roman"><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: FA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US"><em><font size="3"><span style="mso-spacerun: yes"> </span>Dunaliella salina</font></em></span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: FA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">, a green microalga, has been consumed as food and
medicine for a long time.The anti-oxidant and anticancer effects are related to
more production of β- carotene under stress conditions compared with normal
circumstances.The scope of this study was survey of anticancer effect of the
ethanol extract of <i>Dunaliella salina</i> algae <font size="3"><span style="mso-bidi-font-style: italic">under stress(EDSS) and normal (EDSN) conditions </span>on death rate of
<span style="mso-bidi-font-style: italic">skin carcinoma cell line</span> A431 by
MTT [3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide] method in
vitro. Our results showed that the β-carotene amount in stress situation was 1.46
times more than in normal condition.The cytotoxic effect of EDSS exposed to 6.25,
12.5, 25, 50, 100 µg/ml in 6, 24, 48 h which was performed by MTT assay showed
that there was a significant difference in the various time except in 6 h in
both conditions.The results of compared death rate of A431cells exposed to
various concentrations of EDSS and EDSN presented significant difference in 6, 24,
and 48 h after exposure. <span style="mso-spacerun: yes"> </span>The LC</font><sub>50 </sub>of
EDSS in 24 and 48 h were 15.4 and 0.4 µg/ml , respectively and 478.6 and 46.4
µg/ml for EDSN.</span></font>
Dunaliella salina, Anticancer, A431 cell line, MTT assay, β-carotene
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http://jifro.ir/browse.php?a_code=A-10-1-155&slc_lang=en&sid=1
Mo.
Emtyazjoo
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Moghadasi
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Rabbani
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Ma.
Emtyazjoo
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10031947532846007881
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S.
Samadi
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No
N.
Mossaffa
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10031947532846007883
No