Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1401
12
1
gregorian
2023
3
1
22
2
online
1
fulltext
en
Research Article: Tracing molecular adaptation of mudskippers from water to land transition: Insight from the molecular dynamics simulation of collagen type-I
Biotechnology
Biotechnology
پژوهشي
Orginal research papers
<span style="font-size:11pt"><span style="line-height:120%"><span sans-serif="" style="font-family:Calibri,"><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Molecular adaptation of mudskippers to land living is undoubtedly a complex multifactorial phenomenon, and the skin is one of the most influential factors.</span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> This study explores the behavior of collagen type-I (CT1) from the structural and functional point of view using an in-silico approach and discusses its possible role in the adaptation to aerial respiration. </span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">The approach was started by finding the CT1 triple helix gene and protein of <i>Boleophthalmus pectinirostris</i>, <i>Periophthalmus magnuspinnatus</i>, and <i>Danio rerio </i>(as the reference sample), followed by homology modeling and 100 ns molecular dynamics simulation. Subsequently, the trajectories were analyzed for RMSD, RMSF, Rg, H-bond, and SASA.</span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> The results all together showed that the flexibility of the CT1 of <i>Danio rerio</i> is considerably greater than<i> Boleophthalmus</i>, and the latter is more flexible than <i>Periophthalmus</i>. This difference in flexibility is also evident in the morphology of their skin since Zebrafish's skin is more elastic than the two mudskippers. The skin of <i>Boleophthalmus </i>is as well slightly softer than <i>Periophthalmus</i>. Our findings demonstrate that the more terrestrial species have the less flexible CT1 in the skin that saves them from desiccation. However, the skin structure of mudskippers makes a balance between the capability of respiration and saving them from desiccation. The results reveal one of the phenotypic adaptations of mudskippers for living on land, which is congruent with the morphological and physiological distinctive features. </span></span></span></span></span></span></span>
Mudskipper, Collagen, Molecular Dynamics, Water to Land Transition
472
486
http://jifro.ir/browse.php?a_code=A-10-2357-3&slc_lang=en&sid=1
M.
Lagzian
milad.lagzian@gmail.com
100319475328460047797
100319475328460047797
No
Department of Biology, Faculty of sciences, University of Sistan and Baluchestan, Zahedan, Iran
M.
Ghanbarifardi
mehdi.ghanbarifardi@science.usb.ac.ir
100319475328460047798
100319475328460047798
Yes
Department of Biology, Faculty of sciences, University of Sistan and Baluchestan, Zahedan, Iran