en aquaculture aquaculture پژوهشي Orginal research papers <span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><span lang="TR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Sponge-associated bacteria have a special position in marine biotechnology due to their unique biological activities. The main objective of this study was to isolate and identify the bacteria associated with sponges around Qeshm Island, Iran, which inhibit the activity of alpha-glucosidase and alpha-amylase enzymes by the produced metabolites. Samples were collected from sponge species living in the study area, including the genera <i>Haliclona</i> and <i>Niphates</i>. Isolation was performed using culture-dependent techniques</span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">.</span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> A total of 155 bacterial isolates were collected. The diversity pattern of bacteria in the sponge samples showed that the <i>Vibrio</i> and <i>Bacillus</i> constituted the predominant bacterial population. The assessment of alpha-glucosidase inhibitory activity of metabolites extracted from the isolated bacteria showed that 6 isolates could inhibit the enzyme activity with IC₅₀ values </span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:107%"><span cambria="" math="" style="font-family:"><span style="color:black">​​</span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">ranging from 153.5 to 495.4 &mu;g/ml, while 9 bacterial isolates inhibited the activity of alpha-amylase enzyme in IC₅₀ values </span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:107%"><span cambria="" math="" style="font-family:"><span style="color:black">​​</span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">at the range of 112.9 to 670.9 &mu;g/ml. The cytotoxic activity of the metabolites extracted on human umbilical cord endothelial cells showed the toxicity of the three extracts at effective concentrations, while seven isolates showed no toxicity. Genetic identification indicated 97% to 100% similarity of the potent isolates with the NCBI gene bank including <i>Bacillus pumilus, Bacillus safensis, Vibrio alginolyticus, Pseudomonas stutzeri, Vibrio parahaemolyticus, Pseudomonas lurida, Bacillus tequilensi, </i>and<i> Streptomyces enissocaesilis.</i> The results of this study provided a new understanding of the diversity pattern of cultivable sponge-associated bacteria and their inhibitory activity on alpha glucosidase and alpha amylase enzymes.</span></span></span></span></span></span></span> Alpha-amylase, Alpha-glucosidase, Anti-diabetic, Persian Gulf, Secondary metabolites, Sponge-associated bacteria 511 525 http://jifro.ir/browse.php?a_code=A-10-2848-5&slc_lang=en&sid=1 A. Ansarizadeh atefehansarizadeh@gmail.com 100319475328460048997 100319475328460048997 No Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran F. Kafilzadeh kafilzadeh@jia.ac.ir 100319475328460048998 100319475328460048998 Yes Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran S. Tamadoni Jahromi stamadoni@gmail.com 100319475328460048999 100319475328460048999 No Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Abbas, Iran M. Kargar kargar@pel.iaun.ac.ir 100319475328460049000 100319475328460049000 No Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran M. Gozari gozari2020@gmail.com 100319475328460049001 100319475328460049001 No Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran