en aquaculture aquaculture پژوهشي Orginal research papers <p><span style="font-size:12pt"><span style="font-family:Cambria,serif"><span style="color:black">Acid soluble collagen (ASC) from the skin (ASC-S) and bone (ASC-B) of shabout (<em>Arabi</em><em>barbus</em> <em>grypus</em> Heckel, 1843) were isolated and characterized. Both ASC-S and ASC-B from shabout contained glycine as the major amino acid and high amount of proline (Pro), hydroxyproline (Hyp), alanine and glutamic acid. On the basis of dry weight, yields of ASC-S and ASC-B were 6.79 and 2.57%, respectively. Furthermore, fourier transform infrared spectroscopy (FTIR) proved that both collagens were integrated and native. The denaturation temperature of ASC-S and ASC-B were 31.59 and 32.25&deg;C, respectively. Additionally, the results of X-ray diffraction (XRD) proved that the two products retained their helical structures. These collagens had prominent absorptions at 230 nm by UV-Vis spectra. Additionally, the scanning electron microscope (SEM) studies have shown that ACS-S and ASC-B are porous and exhibited fibrous nature. According to the UV&ndash;Vis and FTIR results, extracted collagens were characterized as Type I collagen based on their amino acid profile. In the current study, the total amount of amino acid (Pro+Hyp) was 19.28% and 19.55% for ASC-S and ASC-B, respectively. The results of the current study suggested that the collagen isolated from shabout can potentially be an alternative source for use in the food, pharmaceutical and biomedical industries.</span></span></span></p> Shabout (Arabibarbus grypus), Characterization, FTIR, SEM skin, bone, Type I collagen 671 687 http://jifro.ir/browse.php?a_code=A-10-4080-1&slc_lang=en&sid=1 M. Göçer mmgocer@gmail.com 100319475328460046370 100319475328460046370 Yes Department of Aquaculture and Fisheries Program, Kahta Vocational Training School, Adıyaman University, Adıyaman, Turkey