en Biotechnology Biotechnology پژوهشي Orginal research papers <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Marines are unique resource that provides a diverse array of natural products, primarily from invertebrates such as sponge. As infectious diseases evolve and develop resistance to existing pharmaceuticals, these ecosystems provide novel leads against microbial, cancer, and viral diseases. The purpose of this study is to investigate antimicrobial and antifungal activities of derivatives of phthalates extracted from <i>Haliclona</i> </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">(<i>Soestella</i>)<i> </i></span></span></span><i><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">caerulea</span></span></span></i><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> on some pathogenic organisms. S</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">ponge samples from Larak Island were collected by divers at a depth of 10 meters. After that, marine sponge extract collection was done by chloroform-methanol extraction as a solvent and through chromatography column and nuclear magnetic resonance (NMR) spectroscopy on the sponge sample, components of dioctyl phthalate </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">(DOTP)</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> and dibutyl phthalate (DBP) were purified and identified. Anti-microbial and antifungal activity of extracted components were investigated by means of bacterial broth dilution method and microdilution broth method and </span></span></span><span style="color:black"><a href="https://www.google.com/search?sxsrf=ALeKk02EVwE0yEJauODF-S1zQJ29VUGHFQ:1622533263498&q=Minimum+Inhibitory+Concentration+abbreviation&spell=1&sa=X&ved=2ahUKEwiC7bm29_XwAhVPXsAKHVEeCI0QkeECKAB6BAgBEC4"><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"><span style="text-decoration:none"><span style="text-underline:none">minimum inhibitor concentration</span></span></span></span></span></a></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> (</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">MIC), </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">minimum bactericide concentration</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> (MBC), and </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">minimum fungal concentration</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> (MFC) were determined. The results indicated that </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">DOTP</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> of <i>H. </i>(<i>Soestella</i>)<i> caerulea</i> was active on <i>Bacillus subtilis</i>, <i>Bacillus cereus</i> bacteria. MBC values of </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">DOTP</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> were determined as 2000 &mu;g/m. DBP of <i>H. </i>(<i>Soestella</i>)<i> caerulea</i> was active on gram positive bacteria. MBC values of </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">DOTP</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> were determined as 1000 &mu;g/m for <i>B. cereus</i> and <i>Staphylococcus aureus</i> and 500 &mu;g/mL for <i>B. subtilis</i>. MFC values of </span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">DOTP</span></span></span><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> and DBP were determined as 1000 &mu;g/m and 2000 for <i>Candida albicans</i>. Hence it is assumed that the sponge </span></span></span><i><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Haliclona </span></span></span></i><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">(<i>Soestella</i>)<i> </i></span></span></span><i><span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">caerulea</span></span></span></i> <span style="font-size:11.5pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">exhibited high antimicrobial activity.</span></span></span></span></span></span></span> Marine sponge, Extraction, natural compounds, Dibutyl phthalate, Dioctyl phthalate, Antibacterial activity, Antifungal activity, Larak Island. 1141 1155 http://jifro.ir/browse.php?a_code=A-10-1886-4&slc_lang=en&sid=1 E. Shafeian elnaz_sh24@yahoo.com 100319475328460046506 100319475328460046506 No Department of Marine Science, Faculty of Natural resources and Environment, Science and Research Branch, Islamic Azad University, Tehran, Iran P. Ghavam Mostafavi mostafavi_pa@srbiau.ac.ir 100319475328460046507 100319475328460046507 Yes Department of Marine Science, Faculty of Natural resources and Environment, Science and Research Branch, Islamic Azad University, Tehran, Iran M. Moridi Farimani m_moridi@sbu.ac.ir 100319475328460046508 100319475328460046508 No Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University A. Mashinchian Moradi ali2m@yahoo.com 100319475328460046509 100319475328460046509 No Department of Marine Science, Faculty of Natural resources and Environment, Science and Research Branch, Islamic Azad University, Tehran, Iran M. Nazemi melikanazemi@yahoo.co 100319475328460046510 100319475328460046510 No Persian Gulf and Oman Sea Ecological Research Center, Iranian Fisheries Science Research Institute (IFSRI). Agricultural Research. Education and Extension Organization (AREEO), Bandar Abbas, Iran.