Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1400
2
1
gregorian
2021
5
1
20
3
online
1
fulltext
en
Research Article: Effects of dietary olive oil and butylated hydroxytoluene (BHT) on growth, blood, and immunity indices in juvenile Persian sturgeon (Acipenser persicus)
aquaculture
aquaculture
پژوهشي
Orginal research papers
The present study aimed to investigate the physiological effects of a natural antioxidant, olive oil, and a synthetic antioxidant, butylated hydroxytoluene (BHT), on growth indices, blood and biochemical parameters, and immunity of farmed Persian sturgeons (<em>Acipenser persicus</em>). Three hundred and fifteen juveniles Persian sturgeon, with a mean weight of 108±0.02 g, were randomly assigned to seven treatments each with three replicates (15 fish in each replicate) following the adaptation to brackish water of the Caspian Sea. The fish in three replicates were fed with diets containing 1%, 3%, and 5% olive oil (only as an antioxidant) and other treatments were fed with diets containing 100, 150, and 200 (mg per kg diet) of BHT. A group of fish was fed with the basal diet (containing no olive oil or BHT) as the control. The fish were fed 2-3 times a day for two months. At the end of the trial, blood sampling and biometry were done to determine the growth indices and blood indices. The results showed that the lowest FCR and the highest SGR, BWI, and GR were found in fish fed with 3% olive oil. The highest hemoglobin (Hb) concentration and the lowest hematocrit (Hct) level were observed in olive oil 1%, whereas the highest Hct was observed in 200 mg/kg BHT. The results indicated that there was no significant difference between experimental treatments and the control group in terms of MCV, MCH, and MCHC. The highest percent of neutrophil and monocyte and the lowest percent of lymphocyte were observed in olive oil 5%. Eosinophils were observed in groups of 3% and 5% olive oil, and 200 mg/kg BHT in diet, and its highest level was related to 3% olive oil. The highest cholesterol and triglyceride levels were observed in 200 and 150 mg/kg BHT in diet, respectively. The results of the study demonstrated that albumin levels significantly increased in 5% and 100 mg/kg BHT in diet compared to the others and the control group (<em>p</em><0.05). The lowest glucose level was found in olive oil 1% and 3% and the highest protein level was observed in 5% olive oil and 100 mg/kg BHT in diet. The results showed that the mean blood plasma IgM of fish in diet containing 5% olive oil was significantly higher than the other treatments and control. In addition, CH50 level in 5% olive oil was significantly higher than the other treatments and control group (<em>p</em><0.05). The study findings generally suggested that the addition of olive oil in the diet of juvenile Persian sturgeon can positively improve their growth indices, blood and biochemical parameters, and immunity.<br>
Olive oil, Butylated hydroxytoluene (BHT), Diet, Growth, Biochemical and blood indices, Persian sturgeon
810
827
http://jifro.ir/browse.php?a_code=A-10-204-25&slc_lang=en&sid=1
E.
Hosseinnia
100319475328460040330
100319475328460040330
No
Fisheries Department, Lahijan Branch, Islamic Azad University, Lahijan, Iran
H.
Khara
h.khara1974@yahoo.com
100319475328460040331
100319475328460040331
Yes
Fisheries Department, Lahijan Branch, Islamic Azad University, Lahijan, Iran
M.
Farokhroz
100319475328460040332
100319475328460040332
No
Fisheries Department, Lahijan Branch, Islamic Azad University, Lahijan, Iran
A.
Yousefi Jourdehi
100319475328460040333
100319475328460040333
No
International Sturgeon Research Institute of the Caspian Sea, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran
R.
Kazemi
100319475328460040334
100319475328460040334
No
International Sturgeon Research Institute of the Caspian Sea, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran