Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1400
2
1
gregorian
2021
5
1
20
3
online
1
fulltext
en
Research Article: Adjuvant effect of Licorice (Glycyrrhiza glabra) extract on the efficacy of lactococcosis vaccine in rainbow trout
(Oncorhynchus mykiss)
fish disease
fish disease
پژوهشي
Orginal research papers
This study aimed to assess the adjuvant effect of Montanide ™ ISA 763 AVG and Licorice extract (<em>Glycyrrhiza glabra</em>) on the immune responses and disease resistance of rainbow trout (<em>Oncorhynchus mykiss</em>) immunized with Formalin inactivated whole cells of Lactococcus garvieae vaccine. Rainbow trout with an average initial weight of 72<span dir="RTL">±</span>3 g were randomLy divided into four experimental treatments: non-vaccinated fish (C), fish immunized with the vaccine without adjuvant (V), fish immunized with vaccine plus Montanide<sup>TM</sup> ISA 763 A VG (VM), and fish immunized with containing Licorice extract (VL). Sixty fish were used for each treatment group in three replicates each with 20 fish. Fish were immunized via intraperitoneal (IP) injection after being anesthetized with clove oil. The results showed that the highest lysozyme activity, antibody titer and hemolytic activity of the alternative complement pathway were obtained in the VM group followed by VL and V groups compared to the control fish at 8 weeks post-vaccination. After challenge through IP route with <em>Lactococcus garvieae</em>, the highest survival rate (96.7%) was observed in VM group, followed by VL (76.7%) and V (76.7%) compared to 20% survival in the control group. In conclusion, the results of this study showed that inclusion of Montanide and lactococcosis vaccine can enhance the potency and efficacy of the vaccine in immunized rainbow trout, but fish immunized with vaccine plus Montanide was superior to fish immunized containing licorice extract<span dir="RTL">.</span><br>
Rainbow trout, Lactococcosis, Vaccine, Montanide, Licorice (Glycyrrhiza glabra), Extract
646
662
http://jifro.ir/browse.php?a_code=A-10-776-9&slc_lang=en&sid=1
L.
Zaheri Abdevand
msoltani@ut.ac.ir
100319475328460040298
100319475328460040298
No
Sicence and Research Barnch, Islamic Azad University
M.
Soltani
mehdi.soltani@morduch.edu.au
100319475328460040299
100319475328460040299
Yes
University of Tehran
Sh.
Shafiei
sshafiei@vet.sku.ac.ir
100319475328460040300
100319475328460040300
No
Faculty of veterinary medicine, university of Shahrekord