en aquaculture aquaculture پژوهشي Orginal research papers Sea <span lang="TR" style="font-size:11.0pt"><span calibri="" style="font-family:"><span style="color:black">cucumber <i>Apostichopus japonicus</i> lives in the lower temperature of the seawater and will enter the state of aestivation when the temperature is high. In recent years, the continuous high temperatures in summer have brought great loss to the production of sea cucumber in north China. To study the epigenetic regulating mechanism of <i>A. japonicus</i> and guide the temperature-controlled culture, the methylation level of <i>A. japonicus</i> genome was detected. Breeding imitation <i>A. japonicus</i> under different temperature conditions for a period of time, the difference of methylation level between the set temperature group and the control group was detected and verified. The methylation levels of <i>A. japonicus</i> in different temperature groups were detected. The methylation sites ranged from 84,766 to 96,225, and the sequencing depth was between 42.33 and 153.21. Under the temperature stress, more than 60% of the differentiated methylated genes in different groups were down-regulated, the HBW group had the most differentiated methylated genes (1,405), and the SBW group had the least differentiated methylated genes (643). Gene function classification and enrichment analysis were carried out, and a total of 3,512 differential genes were found, mostly related to immunity and metabolism. The results showed that temperature can change the gene methylation level of <i>A. japonicus</i>, affecting its expression level, and ultimately changing the metabolic and immune processes of <i>A. japonicus</i>.</span></span></span> Apostichopus japonicus, Temperature stress, Genomic methylation, Methylated differential genes 437 456 http://jifro.ir/browse.php?a_code=A-10-3804-1&slc_lang=en&sid=1 P. Gao 1942085396@qq.com 100319475328460051356 100319475328460051356 Yes Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, 116023, China L. Li 18041560312@163.com 100319475328460051357 100319475328460051357 No Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, 116023, China L. Han 1095009677@qq.com 100319475328460051358 100319475328460051358 No Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, 116023, China Y. Zhang 627360098@qq.com 100319475328460051359 100319475328460051359 No Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, 116023, China Z. Quan 1026450501@qq.com 100319475328460051360 100319475328460051360 No Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, 116023, China B. Ding dingbeichen01@qq.com 100319475328460051361 100319475328460051361 No Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, 116023, China Y. Sun 1757660948@qq.com 100319475328460051362 100319475328460051362 No Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, 116023, China B. Han hanbing@dlou.cn 100319475328460051363 100319475328460051363 No Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, 116023, China J. Ding dingjun19731119@hotmail.com 100319475328460051364 100319475328460051364 No Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian, 116023, China