en Biotechnology Biotechnology پژوهشي Orginal research papers <span style="font-size:11pt"><span style="line-height:120%"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">In the present work, </span></span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">extraction of an</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> endosymbiont from a host of </span></span></span></span><i><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Stichodactyla</span></span></span></span></i><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> <i>haddoni </i>collected from the coast </span></span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">of Hormuz Island (the Strait of Hormuz)</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> and afterwards identification, determination of the optimal cultivation conditions</span></span></span></span> <span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">and then finding out the parameters and how they affect its bioactive compounds</span></span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> were conducted</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">. The sequence of ITS2 region from the endosymbiont of <i>S. haddoni</i> clustered with 17 sequences representing clade C. The sequence was deposited to the GenBank under accession number MT448855. The study results showed that </span></span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">ASP₁₂</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> medium and a temperature of 23&deg;C were optimal to cultivate </span></span></span></span><i><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Symbiodinium</span></span></span></span></i><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> sp. outside its host.</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> Under these conditions, t</span></span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">he highest cell density was 12&times;10⁵ cell ml^-1 and the maximal amount of chlorophyll-a and peridinin were 22.81 and 47.</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">86</span></span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> mgL^-1, respectively. Further cultivation of the obtained strain </span></span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">in a Twin-Layer photobioreactor at light intensities of 50, 100 and 250 &mu;mol photons </span></span></span></span><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">m^-2s^-1</span></span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> for 16 days demonstrated that the biomass content was observed to be 35.72, 54.77 and 57.12 </span></span></span></span><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">g m^-2</span></span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> at 50, 100 and 250 &micro;mol photons</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> m^-2s^-1</span></span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">; the peridinin content was 0.26, 0.63 and 0.79 g m</span></span></span></span>^{<span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">-2</span></span></span></span>}<span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">, </span></span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">respectively</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">; total lipid content was 19.5 and 27.77% of dry weight at 50 and 250 &micro;mol </span></span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black">photons</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:120%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> m^-2s^-1.</span></span></span></span></span></span></span> Endosymbiont, Peridinin, Protein, Chlorophyll-a, Total lipid, Growth condition 688 704 http://jifro.ir/browse.php?a_code=A-10-1371-3&slc_lang=en&sid=1 B. Zarei Darki zarei@mail.ru 100319475328460046371 100319475328460046371 Yes Tarbiat Modares University M. Yaseri Gohari hh.yaseri@gmail.com 100319475328460046372 100319475328460046372 No Tarbiat Modares University H. Tahmasebi hamideh.tahmasebi26@gmail.com 100319475328460046373 100319475328460046373 No Tarbiat Modares University