Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1399
6
1
gregorian
2020
9
1
19
5
online
1
fulltext
en
Isolation and identification of antibacterial steroid compounds from Ulva fasciata in the Persian Gulf
Biotechnology
Biotechnology
پژوهشي
Orginal research papers
Due to numerous reasons, marine-obtained resources have attracted the interest of researchers. One of the subjects attracted the interest of researchers in recent years in the field of biologist marine algae due to their nutritional value, their benefits for health and their biological activities. In this study, the powder was extracted using acetone, then the extract purified by silica gel column chromatography with n-hexane and ethyl acetate combination to isolate the steroid compounds. Isolated compound was run through TLC and sprayed with vanillin-sulphuric acid reagent to detect the steroid compounds and profile of isolated compounds obtained by gas chromatography (GC). Antibacterial activity of steroids extracted of <a href="https://www.google.com/search?rlz=1C1GCEA_enIR825IR825&sxsrf=ACYBGNQZSvzK_q85LPMegGhR6AeYTRVApw:1573463782470&q=Ulvaceae&stick=H4sIAAAAAAAAAOPgE-LUz9U3MEnOK6xUAjPNjEzN4rUss5Ot9JMy83Py0yv184vSE_Myi3Pjk3MSi4sz0zKTE0sy8_OsMjLTM1KLFFBFF7FyhOaUJSanJqYCAJQuXT9eAAAA&sa=X&ved=2ahUKEwjWtsiu6eHlAhVCa1AKHTRqDRMQmxMoATAbegQIDhAU">green</a> algae <em>Ulva fasciata</em> from Qeshm Island in the Persian Gulf were assessed (<em>in vitro</em>). The antibacterial activity of the fractions was determined by Broth Dilution Methods against clinical Gram-negative bacteria: Escherichia coli, <em><em>Pseudomonas aeruginosa</em></em>, <em>Salmonella. typhi</em> and Gram-positive bacteria: <em><em>Staphylococcus </em></em><em><em>aureus</em>, </em><em><em>Bacillus subtilis</em></em>, Bacillus cereus. The steroids; cholest-7-en-3ol and cholestan-3-one, cyclic 1, 2-ethanediyl acetal compounds in column fraction were identified by GC. The results showed the minimum bactericidal concentration (MBC) of the cholest-7-en-3ol and cholestan-3-one, cyclic 1,2-ethanediyl acetal for S. aureus and Bacillus cereus were 500 μg ml<sup>-1</sup>, the MBC of the cholest-7-en-3ol for B. subtilis was 500 μg ml<sup>-1</sup> and 1000 μg ml<sup>-1 </sup>detected for cholestan-3-one, cyclic 1,2-ethanediyl acetal. The compounds had neither antibacterial activity on gram negative bacteria. Based on our results, extracted steroids from <em>U. fasciata</em> can be considered as a source of novel antibiotics.
Green algae, Ulva fasciata, Antibacterial activities, Natural products, Persian Gulf
2384
2393
http://jifro.ir/browse.php?a_code=A-10-3512-1&slc_lang=en&sid=1
R.
Azizi
100319475328460036373
100319475328460036373
No
Department of Hygiene, Science and Research Branch, Islamic Azad University, Tehran
A.A.
Motallebi Moghanjoughi
motalebi@ifro.ir
100319475328460036374
100319475328460036374
Yes
Department of Hygiene, Science and Research Branch, Islamic Azad University, Tehran
M.
Nazemi
100319475328460036375
100319475328460036375
No
Persian Gulf and Oman Sea Ecological Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Iran
M.
Sharif Ruhani
100319475328460036376
100319475328460036376
No
Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extention (AREEO)
M.
Afshar Nasab
100319475328460036377
100319475328460036377
No
Kherad higher education center٫ Bushehr, Iran.