Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1400
6
1
gregorian
2021
9
1
20
5
online
1
fulltext
en
Research Article: Comparative effect of different vaccines on immune-related gene expressions of rainbow trout (Oncorhynchus mykiss) and experimentally infected with Streptococcus iniae and Lactococus garvie
fish disease
fish disease
پژوهشي
Orginal research papers
To understand how different vaccines, i.e., bivalent formalin-inactivated vaccine (<em>Lactococus garvie</em> and <em>Streptococcus iniae</em>), could affect the immune-related gene expressions, the TNF-alpha, IL-1β and IL-8 genes of head kidney was measured in <em>Oncorhynchus mykiss</em>. To address this, 630 juvenile fish weighing 26±3 g were initially (day 0) injected by three different treatments, including control group (normal saline only), AquaVac<sup>TM</sup> Garvetil (Merck) and Iranian vaccine (Jehad-e-Daneshagahi) and followed by bath immersion into the same vaccine on day 30. The head kidney was withdrawn to measure immune-related gene expressions at the beginning of experiment (day 0), and following 14, 30, 45 and 60 days of post-vaccination. In the next step, control and both vaccinated groups were also subjected to either <em>L. garvie</em> or <em>S. iniae </em>challenge<em>.</em> Similar samples were taken immediately following bacteria injection (time= 0) and 12, 48, 72 h, and following 7 and 10 days of challenged test. The real-time PCR indicated up-regulation of all cytokine genes following vaccination at day 14 as compared to initial day (<em>p</em><0.05). In pre-challenged experiment, the higher induction in the level of those genes in the head kidney was related to AquaVac vaccine (<em>p</em><0.05). Injection by <em>S. iniae</em> and <em>L. garviae</em> induced the level of immune-related gene expression in the head kidney within the first few days with higher intensity in case of unvaccinated control group. Although the AquaVac produces higher up-regulation of inflammatory gene expression rather than Iranian vaccine, the immunostimulatory effects of both vaccines is a time-restricted.
Cytokine genes, Pro-inflammatory genes, Bacterial infection, Passive vaccination, Oncorhynchus mykiss
1387
1408
http://jifro.ir/browse.php?a_code=A-10-3304-1&slc_lang=en&sid=1
H.
Khaj
Hosseinkhaj@yahoo.com
100319475328460041332
100319475328460041332
Yes
Razi Vaccine and Serum Research Institute, Agriculture Research Education and Extension Organization, AREEO, Karaj, Iran
M.
Mesbah
m.mesbah@scu.ac.ir
100319475328460041333
100319475328460041333
No
Shahid Chamran University of Ahvaz
M.R.
Tabandeh
m.tabandeh@scu.ac.ir
100319475328460041334
100319475328460041334
No
Shahid Chamran University of Ahvaz
T.
Mohammadian
t.mohammadian@scu.ac.ir
100319475328460041335
100319475328460041335
No
Shahid Chamran University of Ahvaz
M.
Dadar
maryamdadar@yahoo.com
100319475328460041336
100319475328460041336
No
Shahid Chamran University of Ahvaz