Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1401
6
1
gregorian
2022
9
1
21
5
online
1
fulltext
en
Research Article: Effect of extracellular proteins (ECP) on protective efficacy of Yersiniosis vaccine in juvenile rainbow trout
(Oncorhynchus mykiss)
aquaculture
aquaculture
پژوهشي
Orginal research papers
<span style="font-size:11pt"><span style="line-height:110%"><span style="font-family:Calibri,sans-serif"><span lang="TR" style="font-size:12.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Yersiniosis is the second important bacterial infections in coldwater fish culture with significant mortalities and economical losses in the Iranian fish farms. In the present study, the effect of extracellular proteins (ECP) on protective immunity<b> </b>of <i>Y. ruckeri</i> vaccine was evaluated in juvenile rainbow trout (7±1.2 g). For this purpose, 540 specimens of juvenile rainbow trout were randomly divided into 6 groups each in triplicates. Group 1 (G<sub>1</sub>) and Group 2 (G<sub>2</sub>) were orally administrated with formalin killed cells (FKC) and FKC+ECP, respectively. Groups 3 to 5 received ECP, FKC, ECP+FKC via intraperitoneal route, respectively. Group 6 received phosphate buffer saline as the control group. The humoral antibody responses to bacterial antigens were monitored by ELISA. LD<sub>50</sub> of <i>Y. ruckeri</i> was determined used probit method sixty days after vaccination. Then, fish in each treatment were challenged intraperitoneally (I.P) with LD<sub>50</sub> of <i>Y. ruckeri</i> virulent registered strain in Iran (KCW 291153). The ELISA results indicated that ECP could increase the serum ELISA antibody titer as the humoral immune response, but ECP with the FKC could increase antibody levels significantly in serum and intestine mucus. Also survival rates in G<sub>1 </sub>to G<sub>6</sub> were 25, 31.25, 37.5, 56.25, 87.5 and 15 percent, respectively. Among I.P immunized fish the survival rate in G<sub>5</sub> was significantly higher than the other groups. Although in orally vaccinated fish with FKC (G1) survival rate did not show significant difference with the control group, the FKC /ECP group (G<sub>2</sub>) showed a significant increase compared to control group (<i>p</i>=0.039)</span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times=""><span style="color:black">.</span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:110%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> It was concluded that supplementing FKC with ECP increased protective immunity of Yersiniosis vaccine principally in I.P. route. Given the great benefits of the oral vaccine, whole cell/ECP can be considered as a protective antigen to design potential vaccines against this pathogenic micro-organism. </span></span></span></span></span></span></span>
Yersinia ruckeri, Immunity, Rainbow trout, Vaccination
1166
1179
http://jifro.ir/browse.php?a_code=A-10-2288-2&slc_lang=en&sid=1
Z.
Tulaby Dezfuly
z.tulaby@gmail.com
100319475328460046517
100319475328460046517
Yes
Ph.D. student of Aquatic Science, Faculty of Veterinary Medicine, ShahidChamran University of Ahvaz, Ahvaz, Iran
M.
Alishahi
100319475328460046518
100319475328460046518
No
Professor of Department of Clinical Sciences
M.
Ghorbanpoor
100319475328460046519
100319475328460046519
No
Professor of Department of microbiology
M.R.
Tabandeh
100319475328460046520
100319475328460046520
No
Associate professor of Biochemistry and Molecular Department
M.
Mesbah
100319475328460046521
100319475328460046521
No
Professor of Department of Clinical Sciences