other پژوهشي Orginal research papers Skin cancer has been reported as a contemporary malignant cancer. Here, anti-cancer effects of sea cucumber extract (SCE) from <em>Holothuria arenicola</em> have been examined on melanoma cells and compared with imidazole carboxamide (Dacarbazine) as a chemotherapy medication against melanoma and Hodgkin&#39;s lymphoma. MTT assay and morphological analysis were performed to evaluate cytotoxic effects of <em>H. arenicola</em> extract. Also, several methods were exerted to detect cell dying by SCE and imidazole carboxamide. The MTT assay showed that B16F10 cells proliferation was blocked by SCE (IC₅₀=31&micro;g <del cite="mailto:NP" datetime="2018-12-21T17:45">ml</del><ins cite="mailto:NP" datetime="2018-12-21T17:45">m</ins>l^-1) and imidazole carboxamide (IC₅₀=1600 &micro;g <del cite="mailto:NP" datetime="2018-12-21T17:45">ml</del><ins cite="mailto:NP" datetime="2018-12-21T17:45">m</ins>l^-1) in a dose and time dependent manner. Apoptosis induction yield treatment occurred at IC₅₀ concentration of SCE and imidazole carboxamide using DAPI staining, Acridine orange/Propodium iodide, PI flow cytometry and annexin/PI assay. The caspase colorimetric kit indicated that SCE and imidazole carboxamide could induce apoptosis through an intrinsic pathway. Collectively, our findings suggested that the methanolic SCE has more efficient cytotoxicity efficiency compared to imidazole carboxamide. Therefore, SCE may be considered as a futuristic marine natural product regarding prevention or treatment of melanoma malignancy. Holothuroidea, Holothuria arenicola, Skin cancer, Imidazole carboxamide, Marine, Caspase 173 187 http://jifro.ir/browse.php?a_code=A-10-1505-4&slc_lang=other&sid=1 J. Baharara baharara@yahoo.com 100319475328460029797 100319475328460029797 Yes Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran N. Nikdel 100319475328460029798 100319475328460029798 No Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran Kh. Nezhad Shahrokhabadi 100319475328460029799 100319475328460029799 No Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran E. Amini 100319475328460029800 100319475328460029800 No Cellular and Molecular Department, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran Cellular and Molecular Department, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran