Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1399
2
1
gregorian
2020
5
1
19
3
online
1
fulltext
en
Effects of commercial herbal oil mixture on some hematological, biochemical and immunological parameters of rainbow trout (Oncorhynchus mykiss) and its preventive efficacy against Yersinia ruckeri infection
fish disease
fish disease
پژوهشي
Orginal research papers
This study was conducted to evaluate the effects of different levels of a commercial herbal oil mixture (<em>Mix-oil<sup>®</sup></em>) on the hematological, biochemical and immunological responses of rainbow trout as well as the resistance against infection with <em>Yersinia ruckeri</em>. The <em>Mix-oil<sup>® </sup></em>includes, <em>Thymus vulgaris</em>, <em>Origanum vulgare</em> and <em>Eucalyptus </em>sp. essential oils. Experimental diets were supplemented with <em>Mix-oil<sup>®</sup></em> at 0 (control group), 50, 200 and 400 ppm. Fish with an initial weight of 31.0± 0.1 g were divided into four groups and reared for 56 days. The anti-bactericidal activity of <em>Mix-oil<sup>® </sup></em>revealed that the minimum inhibitory concentration (MIC) value for <em>Streptococcus iniae</em>, <em>Aeromonas hydrophila</em> and <em>Lactococcus garviae</em> was 6.25 μl ml<sup>-1</sup> while it was 12.5 μl ml<sup>-1</sup> for <em>Y. ruckeri</em>. Significant improvements in red blood cell count, hematocrit, immunoglobulin, lysozyme, total protein, complement component C3 and C4, and alternative complement pathway (ACH<sub>50</sub>) in fish fed with 400 ppm <em>Mix-oil<sup>®</sup></em> were observed compared with the control group (<em>p</em><0.05). No significant difference was observed between different treatments in terms of MCV, MCH, MCHC levels and hemoglobin percentage aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels (<em>p</em>>0.05). The results suggested that dietary administration of <em>Mix-oil<sup>®</sup></em> at the level of 400 ppm could enhance hematological parameters and immune function of rainbow trout as well as the resistance against <em>Y. rukeri</em>. Thus, using <a name="_Hlk515727322"><em>Mix-oil<sup>®</sup></em> </a>as an immunostimulant is recommended for farmed rainbow trout.
Herbal oil mixture, (Mix-oil®), Non-specific immune responses, Rainbow trout, Yersinia ruckeri.
1304
1318
http://jifro.ir/browse.php?a_code=A-10-2181-3&slc_lang=en&sid=1
A.
Bababaalian Amiri
alirezababaalian@gmail.com
100319475328460034059
100319475328460034059
No
Department of Aquatic Animal Health and Diseases, Science and Research Branch, Islamic Azad University, Tehran, Iran
G.
Azari Takami
Takami85@hotmail.com
100319475328460034060
100319475328460034060
Yes
Department of Aquatic Animal Health and Diseases, Science and Research Branch, Islamic Azad University, Tehran, Iran
M.
Afsharnasab
mafsharnasab@yahoo.com
100319475328460034061
100319475328460034061
No
Department of Aquatic Animal Health and Diseases, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, I.R. Iran
A.
Zargar
azargar@ut.ac.ir
100319475328460034062
100319475328460034062
No
Department of Aquatic Animal Health and Diseases, University of Tehran, Tehran, Iran