en aquaculture aquaculture پژوهشي Orginal research papers <em>Tubifex tubifex </em>is a natural feed for many fish species, which still relies on natural catches. High nutrient contents culture media is needed to increase the availability and quality of <em>T. tubifex</em>. The aim of this study was to investigate growth performance of Nile tilapia (<em>Oreochromis niloticus</em>) larvae fed with <em>T. tubifex</em> cultured using different animal manures. Some profiles of <em>T. tubifex</em> nutrition (N, P, and K), Nile tilapia composition (proximate, essential amino acids, and fatty acids) fed with <em>T. tubifex</em>, Relative Growth Rate (RGR), Survival Rate (SR), biomass, feed intake, protein efficiency ratio, and water quality were analyzed. The results showed that tilapia fed with <em>T. tubifex</em> which cultured using various animal manures had a significant effect (<em>p</em><0.05) on its nutrition, growth, biomass, and feed intake level compared to tilapia fed with <em>T. tubifex</em> which cultured without animal manures. However, it did not have significant effect in survival rate (<em>p</em>>0.05). The best treatment was obtained at <em>T. tubifex</em> cultured using 50 g/L of quail manure + 100 g L^-1 of rice bran + 50 g L^-1 of tofu waste (T₂). Growth rate, Oreochromis niloticus, Tilapia larvae, Tubifex tubifex 2039 2052 http://jifro.ir/browse.php?a_code=A-10-2913-1&slc_lang=en&sid=1 V.E. Herawati vivendar23@gmail.com 100319475328460036127 100319475328460036127 Yes Diponegoro University J. Hutabarat johaneshutabarat@gmail.com 100319475328460036128 100319475328460036128 No Diponegoro University Pinandoyo pinandjaya@yahoo.com 100319475328460036129 100319475328460036129 No Diponegoro University O. Karna Radjasa Ock_radjasa@yahoo.com 100319475328460036130 100319475328460036130 No Diponegoro University