Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1385
10
1
gregorian
2007
1
1
6
2
online
1
fulltext
en
Effects of cortisol on tesicular apoptosis in goldfish (Carassius auratus)
Biology & physiology
Biology & physiology
پژوهشي
Orginal research papers
Apoptosis is a programmed cell death that has been attributed to the action of stress hormones, such as cortisol. We investigated the <em>in vitro</em> effect of cortisol in goldfish (<em>Carassius auratus</em>) mature (GSI: 2.75-4.65) or immature (GSI: 1.94-2.4) testes. Testes were taken out and transferred into culture medium (M 199), cut into 20 mm thick slices and supplemented with fetal bovine serum (FBS), added different doses of cortisol (0, 50, 100, 200, 400, 750 and 800 ng/ml) and incubated for 24h, 48h or 72h at 18°C. Samples were rinsed and homogenized with phosphate buffered saline (PBS) after incubation period. Duplicates or triplicates processed for caspase-3 activity. The results showed a significant increase in caspase-3 activity in immature testes after 72h treatment with 750ng/ml of cortisol (P<0.05), and also at 400ng/ml (P<0.05). In contrast, no significant differences were observed between non-treated and cortisol-treated samples from mature goldfish testes. In this study we have demonstrated, for the first time that the stress hormone cortisol induces apoptosis in the goldfish testis in a stage-specific manner (immature testis).
Apoptosis, Caspase 3, Cortisol, Goldfish, Stress, Testis
1
14
http://jifro.ir/browse.php?a_code=A-10-600-112&slc_lang=en&sid=1
M.
Bahmani
mahmoudbahmani@yahoo.com
100319475328460024256
100319475328460024256
Yes
C.V.
Andreu-Vieyra
100319475328460024257
100319475328460024257
No
H.R.
Habibi
100319475328460024258
100319475328460024258
No