en aquaculture aquaculture پژوهشي Orginal research papers <p style="margin-left:50.4pt;">In the present study, cryopreservation of common carp (<em>Cyprinus carpio</em>) embryos was studied using a stepwise cooling protocol. Embryos at heartbeat stage were selected, placed in 15- ml plastic tubes and stored at -2 &deg;C for 1, 7, 14, and 21 days in 2 different cryopreservation solutions: S1 (methanol + dimethyl sulfoxide + sucrose) and S2 (methanol + propylene glycol + sucrose). Embryo viability was assessed by survival rate, counting live larvae and number of failed eggs under a stereomicroscope.&nbsp; The results showed that storage of common carp embryos was possible for up to 7 days at -2 &deg;C, while the survival did not occur when storage period were 14 and 21 days. The combination of methanol with propylene glycol (S2) gave higher survival rate after 1 and 7 days stored at -2 &deg;C. Further studies are needed in order to extend the storage time and to improve the survival rate for this species.</p> Cryopreservation, Common carp, Cryoprotectant 1036 1045 http://jifro.ir/browse.php?a_code=A-10-2354-1&slc_lang=en&sid=1 S Keivanloo Skeivanloo@yahoo.com 100319475328460031080 100319475328460031080 No Gorgan University of Agricultural Sciences and Natural Resources M Sudagar sudagar_m@yahoo.com 100319475328460031081 100319475328460031081 Yes Gorgan University of Agricultural Sciences and Natural Resources M Mazandarani mazandarani57@gmail.com 100319475328460031082 100319475328460031082 No Gorgan University of Agricultural Sciences and Natural Resources