Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1394
10
1
gregorian
2016
1
1
15
1
online
1
fulltext
fa
Culture and molecular characterization of phages isolated from rainbow trout farms and sewage treatment plants and investigation of their effects on Yersinia ruckeri
fish disease
fish disease
پژوهشي
Orginal research papers
<p dir="ltr" style="text-align: justify;">In the present study bacterioph<a name="_GoBack"></a>ages isolated from rainbow trout farms and sewage treatment plants were genetically identified and their effectiveness on <em>Yersinia ruckeri </em>isolates from clinical cases of red mouth disease was investigated. Fish samples suspected to red mouth disease were collected from rainbow trout farms located in west Azerbaijan. <em>Y. ruckeri</em>, the causative agent of red mouth disease was initially identified using biochemical tests. The biotypes of all <em>Y. ruckeri</em> isolates were determined and their identity was confirmed by employing genus specific primers. Antimicrobial resistance of <em>Y. ruckeri</em> isolates were examined using common antibiotics in use in aquaculture. In order to isolating lytic bacteriophages, environmental samples mainly from rainbow trout farms and sewage treatment plants were collected in a period of six months. Isolated bacteriophages were titrated using two-layer agar method and their bactericidal effects were examined. For molecular characterization of bacteriophages, genomic DNA was extracted. Extracted genomic DNA from bacteriophages was digested using <em>Msp</em>I endonuclease. The results revealed that 4.48% of examined fish were positive for <em>Y. ruckeri.</em> Bacteriophages isolated from urban sewage treatment plants were effective on <em>Y. ruckeri</em> isolates. Maragheh and Urmia sewage treatment plants had the maximum and minimum phage titers, respectively. The genomic DNA of all isolated phages were smaller than genomic DNA of <em>Lambda</em> phage and all examined phages showed similar genomic DNA digestion patterns. It was concluded that sewage treatment plants could be an important source for phages effective on <em>Y. ruckeri </em>and maybe other aquaculture bacterial pathogens. </p>
Bacteriophage, Rainbow trout, Redmouth disease, Wastewater, Yersinia ruckeri
267
280
http://jifro.ir/browse.php?a_code=A-10-1272-166&slc_lang=fa&sid=1
S.
Ahmadpour
100319475328460015266
100319475328460015266
No
K.
Mardani
100319475328460015267
100319475328460015267
No
A.
Tukmechi
a.tukmachi@urmia.ac.ir
100319475328460015268
100319475328460015268
Yes