en fish disease fish disease پژوهشي Orginal research papers <span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: EN-US">Over the past few years, the syndrome of streptococcosis </span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO">has been associated with outbreaks in r<font size="3"><font face="Times New Roman"><span style="COLOR: #231f20">ainbow trout (<i>Oncorhynchus mykiss</i>, Walbaum)</span> and</font></font></span><span style="FONT-FAMILY: "Times New Roman","serif" LETTER-SPACING: 0.05pt COLOR: #231f20 FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: EN-US"> caused significant economic losses in the aquaculture industry in Iran.</span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO"> </span><span style="FONT-FAMILY: "Times New Roman","serif" LETTER-SPACING: 0.15pt COLOR: black FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: EN-US">The main purpose of this work was molecular identification of some causative agents of streptococcosis in rainbow trout. </span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO">A total of 520 <span style="COLOR: #231f20"><font size="3" face="Times New Roman">samples were collected from</font></span></span><span style="FONT-FAMILY: "Times New Roman","serif" LETTER-SPACING: 0.15pt COLOR: black FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: EN-US"> the head</span><span style="FONT-FAMILY: "Times New Roman","serif" COLOR: #231f20 FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO"> kidney of diseased fish (weight, 50^_200g)</span><span style="FONT-FAMILY: "Times New Roman","serif" LETTER-SPACING: 0.15pt COLOR: black FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: EN-US"> in 72 farms of 8 provinces in <place w:st="on"><country-region w:st="on">Iran</country-region></place>, during 2008 to 2009. </span><span style="FONT-FAMILY: "Times New Roman","serif" COLOR: #231f20 FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO">Bacterial isolates representing morphology and biochemical profiles of <i>Streptococcus spp</i></span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO">.<i> </i>were<span style="COLOR: #231f20"><font size="3" face="Times New Roman"> further confirmed by polymerase chain reaction (PCR).</font></span></span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: EN-US"> DNA extraction was carried out from a single colony by using the extraction promega kit following the conditions described by the supplier. The </span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO">PCR assay was developed based on the </span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: EN-US">16S rRNA and glucose kinase genes </span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO">of <i>Streptococcus</i> spp. for the rapid and specific detection and identification of this pathogen from different sources</span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: EN-US">. </span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: KO">A</span><span style="FONT-FAMILY: "Times New Roman","serif" FONT-SIZE: 12pt mso-fareast-font-family: 'Times New Roman' mso-bidi-language: AR-SA mso-ansi-language: EN-US mso-fareast-language: EN-US">pproximately 40% of specimens were infected to <i>Streptococcus</i> <i>spp</i>. Consequently, five pathogenic species have been identified, including <i>S. iniae</i> in Fars province, <i>S. faecium</i> in Mazandaran province, <i>S. agalactiae</i> in Gilan and Mazandaran provinces, <i>S. dysgalatiae</i> in Lorestan, Kohgiluyeh and Boyerahmad, Gilan and Kermanshah provinces and <i>S. uberis,</i> which was common in all provinces (except Mazandaran and Lorestan). The dominant species (based on important species index) were <i>S. uberis</i>, <i>S. dysgalactiae</i> and <i>S. agalactiae</i>, respectively.</span> Iran, Streptococcosis, Rainbow trout, Syndrome, PCR, DNA 109 122 http://jifro.ir/browse.php?a_code=A-10-1-65&slc_lang=en&sid=1 R. Pourgholam r_pourgholam@yahoo.com 10031947532846001096 10031947532846001096 Yes F. Laluei 10031947532846001097 10031947532846001097 No A. A. Saeedi 10031947532846001098 10031947532846001098 No A. Zahedi 10031947532846001099 10031947532846001099 No R. Safari 10031947532846001100 10031947532846001100 No M. J. Taghavi 10031947532846001101 10031947532846001101 No H. Nasrollhzadeh Saravi 10031947532846001102 10031947532846001102 No H. Pourgholam 10031947532846001103 10031947532846001103 No