Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1389
2
1
gregorian
2010
5
1
9
2
online
1
fulltext
en
Isolation of common carp ovarian follicular cells and evaluation of their endocrine activity in primary cell culture
پژوهشي
Orginal research papers
<p> To study viability and activity of isolated common carp, <i>Cyprinus carpio</i>, ovarian follicular cells (granulosa and theca cells), 17- α -Hydroxy progesterone (17 α -OHP) and 17 β -Estradiol (E<sub>2</sub>) levels were estimated in the culture media of cultivated carp ovarian follicular cells, using radioimmunoassay (RIA). Oocytes were isolated from the ovaries of female carp. Interstitial tissue was manually removed in order to obtain single oocytes surrounded only by the follicular envelope. Such a preparation was trypsinized at room temperature. Follicular cell suspension contained both cells and cell clumps. It was the mixture of theca (T) and granulosa (G) cells. The cells were grown as monolayer in 24-well microplates in M199 medium supplemented with FBS. Culture media were analyzed for estrogen and progesterone content by appropriate radioimmunoassay. Trypsinization of ovarian follicles resulted in the formation of the cell suspension which contained a mixture of G and T cells. The follicular cells attached to glass and grew well during culture period. E<sub>2 </sub>was the main steroid hormone secreted by cultivated cells. Estrogen secretion increased by 415.52 ± 25 pg/mL at the first 3 days up to 530.25 ± 55.8 pg/ml on day 5 and it didn't change significantly until the end of the experiment. 17 α -OHP secretion, however, gradually increased from 23.84 ± 8.2 pg/ml at the beginning of culture up to 35.76 ± 5.4 pg/ml at the end of cultivating. As the result of the present study the fish follicular cells grown in tissue culture were steroidogenically active cells as expressed by the secretion of E<sub>2</sub> and P<sub>4</sub> and The E<sub>2</sub> was a dominant hormone secreted by isolated follicular cells, which it correlated closely with vitellogenes stage </p>
Granulosa cells, Theca cells, Steroid hormones, Cyprinus carpio, Cell culture.
305
314
http://jifro.ir/browse.php?a_code=A-10-1-10&slc_lang=en&sid=1
N.
Salamat
salamatnegin@yahoo.com
100319475328460015
100319475328460015
Yes
N.
Erfani Majd
100319475328460016
100319475328460016
No
M.
Hashemitabar
100319475328460017
100319475328460017
No
M.
Mesbah
100319475328460018
100319475328460018
No
A.
Ahangarpoor
100319475328460019
100319475328460019
No