AU - Soltani, M. AU - Khazraeenia, S. AU - Sepehri, H. TI - Some effects of experimental acidification on phenoloxidase, trypsin and lysozyme activities in freshwater crayfish (Pontastacus leptodactylus) PT - JOURNAL ARTICLE TA - IFRO JN - IFRO VO - 7 VI - 1 IP - 1 4099 - http://jifro.ir/article-1-3084-en.html 4100 - http://jifro.ir/article-1-3084-en.pdf SO - IFRO 1 AB  - Levels of phenoloxidase (Po), trypsin and lysozyme activities were determined following 48 hours exposing freshwater crayfish (Pontastacus leptodactylus) weighing 59.8±3.4g to different low pH values of 6.5, 5.5, 4 and 2.5 at 20-23˚C. Significant decrease were found (p<0.01) in levels of Po and trypsin activities in crayfish haemocyte lysate supernatant (HLS) at pHs 5.5 and 4 compared to the control group (pH 8). Also, plasma lysozyme activity showed a significant increase in water with pHs of 6.5, 5.5 and 4 (p<0.01). Furthermore, measurement of calcium and magnesium concentrations in sera samples showed a significant decrease for calcium ion at pHs 5.5 and 4, while magnesium concentration was significantly decreased only at pH 4 compared to control group (p<0.05). Crayfish exposed to pH 2.5 died within 24 hours post-exposure. CP - IRAN IN - LG - eng PB - IFRO PG - 87 PT - Orginal research papers YR - 2008