AU - Sembiring, S.B.M. AU - Giri, N.A. AU - Pratiwi, R. AU - Haryanti, H. AU - Hadisusanto, S. TI - Research Article: Effects of dietary protein levels on growth performance, amino and Fatty acids of juvenile sandfish, Holothuria scabra (Jaeger, 1833) PT - JOURNAL ARTICLE TA - IFRO JN - IFRO VO - 21 VI - 6 IP - 6 4099 - http://jifro.ir/article-1-5104-en.html 4100 - http://jifro.ir/article-1-5104-en.pdf SO - IFRO 6 ABĀ  - The experiment was carried out for 120 days to determine the optimal dietary protein requirement for the growth of sandfish, Holothuria scabra. The experiment used 12 plastic containers sized 59×47×37 cm, and each container stocked 30 individual hatchery-produced sandfish juveniles (initial weight= 2.70±0.01 g). Experimental diets with different levels of protein, i.e., 10% (A), 20% (B), and 30% (C) as a treatment; it was as dry pellet with 3 mm diameters. Sandfish were fed with experimental diets once in the afternoon at 2 % biomass. Results of the experiment showed that sandfish fed a 20% protein diet has the highest final weight (5.10±0.02 g), specific growth rate (SGR; 0.53±0.01 % day-1), and feed efficieny (FE; 62.48±0.13) and is significantly different compared to other treatments (p<0.05). On the other hand, feeding with different dietary protein levels did not influence the survival rate greatly (p>0.05), ranging from 85.00-95.83%. The total amino acid content of sandfish juveniles in treatment B was highest (6.62%) and followed by C (6.27%) and A (4.75%). Likewise, the highest fatty acid content was also found in treatment B (0.440%) followed by treatments A and C of 0.416%; 0.332% respectively. Results of the present study found the best feed was treatment B at 20 % protein content. Moreover, based on statistical analyses, it is suggested that dietary protein requirement for maximum growth of juvenile sandfish is 16%. CP - IRAN IN - Jl. Raya Gilimanuk Singaraja, Dusun Gondol, Desa Penyabangan, kecamatan Gerokgak, Kabupaten Singaraja, Bali, Indonesia LG - eng PB - IFRO PG - 1447 PT - Orginal research papers YR - 2022