AU - Ghorbani Vaghei, R. AU - Shenavar Masoule, A.R. AU - Kazemi, R. AU - Jalilpour, J. AU - Hoseinpour, A. AU - Sayed Hassani, M.H. AU - Alizadeh Rudposhti, M. TI - Research Article: Effects of adding two native bacterial strains (Lactococcus lactis and Weissella confusa) on growth performance, immune indices, and intestinal flora of juvenile great sturgeon (Huso huso) PT - JOURNAL ARTICLE TA - IFRO JN - IFRO VO - 20 VI - 4 IP - 4 4099 - http://jifro.ir/article-1-4234-en.html 4100 - http://jifro.ir/article-1-4234-en.pdf SO - IFRO 4 ABĀ  - This study was carried out to determine effects of diets supplemented with two bacterial strains (Lactococcus lactis and Weissella confusa) on the growth performance, immune indices and intestinal microflora of great sturgeon juveniles. At the beginning of the feeding trial, the mean weight (±SD) of the fish was 79.44±3.18 g. At random, 15 fish were stocked per each fiberglass tank (1m×1m×0.5m) containing 300 L freshwater. The diets were prepared through spraying 50 ml bacterial suspensions containing 150, 300, and 450 mg of the bacterial strains per kg of pelleted diets to make certain concentrations 1.5×109 cfu/g (T1), 3×109 cfu/g (T2), 4.5×109 cfu/g (T3). The blood neutrophils in the T1 and T2 significantly increased as compared to the control group and T3. Lymphocytes in the control and T3 were significantly more than T1 and T2. However, eosinophils showed no change between the fish fed with the supplemented diets and control group. Monocytes in T3 considerably decreased when compared to T1, T2, and control. IgM and C3 in the experimental treatments were significantly higher than the control. Lysozyme, C4, and ACH50 in T1 an T2 were significantly higher than T3 and control. Colony count of lactic acid bacteria in the intestine of fish in T1 and T2 was significantly higher than the control and T3 groups. Colony count of the aerobic and facultative anaerobic bacteria in the intestine of fish in the medium of TSA in control was significantly more than T1 and T2. Since the TSA medium is a kind of non-selective environment and provides sufficient nutrients for a wide range of microorganisms, the medium indicated that intestinal microflora condition was worse in the control fish. The growth performance indices (weight gain, biomass increase, specific growth rate, daily weight gain, and condition factor) demonstrated no significant difference between treatments and control. There was no significant difference in term of FCR between control, T1, and T3. Overall, it can be stated that the two bacterial strains could induce favorable influence on intestinal microflora, immune indices, biochemical parameters, and growth performance at two levels of 150 mg (T1) and 300 mg (T2) especially in the T2. CP - IRAN IN - Guilan Province- Rasht-Shahrestan- Postal Cod: 4338133986 LG - eng PB - IFRO PG - 1206 PT - Orginal research papers YR - 2021