[صفحه اصلی ]   [Archive]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
:: دوره 14، شماره 2 - ( 1394 ) ::
جلد 14 شماره 2 صفحات 446-456 برگشت به فهرست نسخه ها
Molecular cloning and recombinant expression of the VP28 (wsv421 gene) from Iranian white spot syndrome virus isolate
چکیده:   (4074 مشاهده)
White spot syndrome virus (WSSV) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including Iran. In the present study, a pair of primers was designed according to the sequence of VP 28 gene of WSSV in the GenBank. VP28 gene from an Iranian WSSV isolate (IrVP28) was cloned, sequenced and expressed in Escherichia coli BL21(DE3) strain in order to produce VP28 protein using PET28a expression vector. The expression resulted in a protein of about 30kDa, which was purified under denaturing conditions, resulting in a highly purified final IrVP28 preparation. The obtained recombinant protein can be used in several biotechnology applications, such as production of monoclonal antibodies which could be used in development of diagnostic tools and potential oral vaccination of shrimp with vaccines consisting of VP28 proteins.
متن کامل [PDF 366 kb]   (1232 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: Biotechnology
دریافت: 1391/11/21 | پذیرش: 1393/9/8 | انتشار: 1394/4/7
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA


XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hosseini D. Molecular cloning and recombinant expression of the VP28 (wsv421 gene) from Iranian white spot syndrome virus isolate . IJFS. 2015; 14 (2) :446-456
URL: http://jifro.ir/article-1-891-fa.html

Molecular cloning and recombinant expression of the VP28 (wsv421 gene) from Iranian white spot syndrome virus isolate . مجله علوم شیلاتی ایران. 1394; 14 (2) :446-456

URL: http://jifro.ir/article-1-891-fa.html



دوره 14، شماره 2 - ( 1394 ) برگشت به فهرست نسخه ها
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 4142